펄스형 전자기장(PEMF) 자극을 이용한 DNCB로 유발된 아토피성 피부염 개선 효과 (Effect on Improvement of Atopic Dermatitis Induced by DNCB using PEMF(Pulsed Electromagnetic Fields) Stimulation)

서지정보

· 발행기관 : 대한의용생체공학회
· 수록지 정보 : 의공학회지 / 42권 / 2호 / 48 ~ 54페이지
· 저자명 : 김준영, 이지영, 이자우, 이수경, 박창순, 양세정, 이용흠

초록

Objective: The purpose of this study is to evaluate the improvement of atopic dermatitis induced by DNCB using pulsed electromagnetic fields (PEMF) stimulation through animal models. Methods: Twenty 6-week Hairless Mouse (SKH1) models were randomly divided into normal groups (n=5), PEMF 15 Hz groups (n=5), PEMF 75 Hz groups (n=5), and Sham groups (n=5). To induce uniform atopic dermatitis, the DNCB(2,4-Dinitrochlorobenzene) solution was applied for 6 weeks. During the process of skin inflammation, sensory evaluations were conducted every 3~4 days to grade symptoms of atopic dermatitis. Following the onset of atopic dermatitis symptoms, the PEMF stimulation (15 mT) in the PEMF group (15 Hz & / 75 Hz) was stimulated for 8 hours per day for 1 week.
After all experiments, the dosal skin tissue was sectioned at 4 μm, and after H&E Staining, the skin tissue was observed through the light microscopy to analyze the skin lesion size. Results: As a result of the sensory evaluation, the difference in average sensory evaluation scores between PEMF groups (15 Hz & 75 Hz) was 0.4 points, indicating a low statistical significance. Between the PEMF 15 Hz Group and the Sham Group, the difference in average sensory evaluation scores was reduced to 1.6 points, resulting in high statistical significance (P<0.05) and the best effect.
As a result of analyzing the size of the lesion, the difference in size of the lesion area between PEMF groups (15 Hz & 75 Hz) was 807 pixels, indicating a low statistical significance. Between the PEMF group (15 Hz & 75 Hz) and the Sham group, the size difference of the lesion area was 12946 pixels, 13753 pixels, respectively, indicating high statistical significance (P<0.001). It means that inflammation has improved in dorsal skin tissue. Conclusions: This study confirms that PEMF (15 Hz / 75 Hz, 15 mT) stimulation can improve symptoms of DNCB-induced atopic dermatitis.

영어초록

Objective: The purpose of this study is to evaluate the improvement of atopic dermatitis induced by DNCB using pulsed electromagnetic fields (PEMF) stimulation through animal models. Methods: Twenty 6-week Hairless Mouse (SKH1) models were randomly divided into normal groups (n=5), PEMF 15 Hz groups (n=5), PEMF 75 Hz groups (n=5), and Sham groups (n=5). To induce uniform atopic dermatitis, the DNCB(2,4-Dinitrochlorobenzene) solution was applied for 6 weeks. During the process of skin inflammation, sensory evaluations were conducted every 3~4 days to grade symptoms of atopic dermatitis. Following the onset of atopic dermatitis symptoms, the PEMF stimulation (15 mT) in the PEMF group (15 Hz & / 75 Hz) was stimulated for 8 hours per day for 1 week.
After all experiments, the dosal skin tissue was sectioned at 4 μm, and after H&E Staining, the skin tissue was observed through the light microscopy to analyze the skin lesion size. Results: As a result of the sensory evaluation, the difference in average sensory evaluation scores between PEMF groups (15 Hz & 75 Hz) was 0.4 points, indicating a low statistical significance. Between the PEMF 15 Hz Group and the Sham Group, the difference in average sensory evaluation scores was reduced to 1.6 points, resulting in high statistical significance (P<0.05) and the best effect.
As a result of analyzing the size of the lesion, the difference in size of the lesion area between PEMF groups (15 Hz & 75 Hz) was 807 pixels, indicating a low statistical significance. Between the PEMF group (15 Hz & 75 Hz) and the Sham group, the size difference of the lesion area was 12946 pixels, 13753 pixels, respectively, indicating high statistical significance (P<0.001). It means that inflammation has improved in dorsal skin tissue. Conclusions: This study confirms that PEMF (15 Hz / 75 Hz, 15 mT) stimulation can improve symptoms of DNCB-induced atopic dermatitis.

참고자료

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