六味地黃湯이 아토피피부염을 유발한 NC/Nga mouse에 미치는 영향 (The Effects of Yukmijihwang-tang on Atopic Dermatitis of NC/Nga Mouse)

서지정보

· 발행기관 : 대한한방안이비인후피부과학회
· 수록지 정보 : 한방안이비인후피부과학회지 / 22권 / 1호 / 120 ~ 132페이지
· 저자명 : 조재훈, 김윤범

초록

Objective : Yukmijihwang-tang is one of the important medicines for blood- deficiency and yin-deficiency.
Atopic dermatitis usually shows dampness-heat pattern in its acute stage and blood-deficiency or
yin-deficiency pattern in its chronic stage. Therefore, I hypothesized that Yukmijihwang-tang is effective on
atopic dermatitis and investigated the effects of Yukmijihwang-tang on NC/Nga mice's atopic dermatitis
induced by DNCB.
Methods : The NC/Nga mice with atopic dermatitis were divided into four groups: three experimental
groups and one control group. The experimental groups were respectively put on 2.5g(YM-2.5), 5g(YM-5)
and 10g(YM-10) of Yukmijihwang-tang extract per their weight once a day for 10 days while the control group
was fed normal saline. After 10 days, I measured TEWL(transepidermal water loss), observed scratching behaviors,
conducted a skin biopsy and checked levels of Total IgE, IL-4 and IFN-ɤ on NC/Nga mice.
Results : 1. Yukmijihwang-tang significantly suppressed skin dryness. In particular, YM-5 and YM-10 had
better skin hydration than YM-2.5.
2. Yukmijihwang-tang significantly suppressed pruritus while there was no significant difference
among the experimental groups.
3. Yukmijihwang-tang retained skin structure(epidermis, dermis and subcutaneous fat).
4. Yukmijihwang-tang reduced Total IgE level while there was no significant difference between
the control group and the experimental groups.
5. Yukmijihwang-tang did not reduce IL-4(Th2 cytokine) level.
6. Yukmijihwang-tang significantly reduced IFN-ɤ(Th1 cytokine) level. In particular, YM-2.5 and
YM-5 had lower IFN-ɤ level than YM-10.
Conclusion : The results suggest that Yukmijihwang-tang suppresses skin dryness and pruritus, retains skin structure
and is effective on chronic atopic dermatitis which is associated with Th1 cytokines in the immune response.

영어초록

Objective : Yukmijihwang-tang is one of the important medicines for blood- deficiency and yin-deficiency.
Atopic dermatitis usually shows dampness-heat pattern in its acute stage and blood-deficiency or
yin-deficiency pattern in its chronic stage. Therefore, I hypothesized that Yukmijihwang-tang is effective on
atopic dermatitis and investigated the effects of Yukmijihwang-tang on NC/Nga mice's atopic dermatitis
induced by DNCB.
Methods : The NC/Nga mice with atopic dermatitis were divided into four groups: three experimental
groups and one control group. The experimental groups were respectively put on 2.5g(YM-2.5), 5g(YM-5)
and 10g(YM-10) of Yukmijihwang-tang extract per their weight once a day for 10 days while the control group
was fed normal saline. After 10 days, I measured TEWL(transepidermal water loss), observed scratching behaviors,
conducted a skin biopsy and checked levels of Total IgE, IL-4 and IFN-ɤ on NC/Nga mice.
Results : 1. Yukmijihwang-tang significantly suppressed skin dryness. In particular, YM-5 and YM-10 had
better skin hydration than YM-2.5.
2. Yukmijihwang-tang significantly suppressed pruritus while there was no significant difference
among the experimental groups.
3. Yukmijihwang-tang retained skin structure(epidermis, dermis and subcutaneous fat).
4. Yukmijihwang-tang reduced Total IgE level while there was no significant difference between
the control group and the experimental groups.
5. Yukmijihwang-tang did not reduce IL-4(Th2 cytokine) level.
6. Yukmijihwang-tang significantly reduced IFN-ɤ(Th1 cytokine) level. In particular, YM-2.5 and
YM-5 had lower IFN-ɤ level than YM-10.
Conclusion : The results suggest that Yukmijihwang-tang suppresses skin dryness and pruritus, retains skin structure
and is effective on chronic atopic dermatitis which is associated with Th1 cytokines in the immune response.

참고자료

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