Lilium Asiatic hybrid ‘Dreamland’의 약배양에 미치는 화분 발육단계와 온도 전처리의 영향 (Effect of Developmental Stage of Pollen Grain and Temperature Pretreatment on Anther Culturein Lilium Asiatic Hybrid ‘Dreamland’)

서지정보

· 발행기관 : 한국화훼학회
· 수록지 정보 : 화훼연구 / 16권 / 1호 / 12 ~ 16페이지
· 저자명 : 박영애, 정재동, 임기병, 황윤정, 박인숙, 서동희, 전수민, 여금복, 이가영

초록

나리(Lilium Asiatic hybrid ‘Dreamland’) 의약배양을통하여반수체를얻어체계적인육종방법및유전
양식을규명할목적으로배양효율에미치는화분발육단계와온도전처리가미치는영향에대한실험결과는다음과같다. ‘Dreamland’ 의감수분열중인약을현미경적방법으로검경하여본결과화뢰의길이가
23.0-24.9mm 는 tetrad 단계이었고 25.0-26.9mm 는uninucleate 단계, 27.0-28.9mm 에서는 binucleate 단계임을확인할수있었으며, 저온및고온온도처리결과는전체적으로캘러스및배발생율은저조하였고특이한점은발견할수없었다. Microgametogenesis 단계별로약배양효율을조사한결과, late uninucleate-early binucleate 단계인 23.0-28.0mm에서 17.8% callus가유도되었고, 식물체재분화율도 6.7%로나타나적정배양 stage 임을확인할수있었다. 유기된캘루스를 picloram 과 zeatin 을혼용한 MS 배지에이식하여 25˚C에서배양하여식물체를얻었다.

영어초록

his study was carried out to produce haploid plants to verify a systematic breeding program and genetic analysis. Effect of developmental stage of pollen grains and pre-treatment temperature on pro­duction of haploid plants was investigated. Microscopic investigation of the explants (Lilium Asiatic hybrid ‘Dreamland’) revealed that the length of flower bud at 23.0-24.9, 25.0-26.9, and 27.0-28.9 mm long coin­cided with tetrad, uninucleate, and binucleate, respectively. When the efficiency of the anther culture from microgametogenetic stages was tested, late uninucleate to early binucleate stage, having the length of 23.0 to
28.0 mm long flower bud, was the best. The frequencies of the callus induction and plant regeneration from the stage mentioned above were 17.8 and 6.7%, respectively. When calli were cultured on the MS medium containing picloram and zeatin at 25oC, shoots were obtained. Roots of regenerated plantlets were con­firmed as haploid through an microscopic observation.

참고자료

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