야관문 에탄올 추출물의 항산화 및 Metalloproteinase 저해 활성 (Antioxidant and Metalloproteinase Inhibitory Activities of Ethanol Extracts from Lespedeza cuneata G. Don)

서지정보

· 발행기관 : 한국환경농학회
· 수록지 정보 : 한국환경농학회지 / 36권 / 4호 / 263 ~ 268페이지
· 저자명 : 신용하, 송창길

초록

야관문의 항산화 활성과 항주름 활성을 조사하기 위하여30% 에탄올 추출물과 각종 용매 분획물을 이용하여 DPPH 및 ABTS radical 소거활성과 matrix metalloproteinase MMP1과 MMP3 생성억제 효과를 조사하였다. 에탄올 추출물과 각종 용매 분획물 중 에틸아세테이트 분획물에서 페놀및 플라보노이드 함량이 가장 높았다. 야관문 추출물의 항산화 활성과 피부 주름을 유발하는 MMP1, MMP3의 생성을억제 효과는 페놀 및 플라보노이드 함량과 높은 상관관계를보였으며, 페놀과 플라보노이드 함량이 가장 높은 에틸아세테이트 분획물의 항산화 활성과 MMPs 생성 억제 효과가 가장높았다.

영어초록

BACKGROUND: Lespedeza cuneata G. Don is a wellknown medicinal plant. In this study, the biological activities of L. cuneata extracts were investigated.
METHODS AND RESULTS: L. cuneata shoot was extracted with 30% ethanol and further fractionated with organic solvents. Total phenolic and flavonoid content, antioxidant activity, andmatrixmetalloproteinase inhibition effect of the extract and fractions were measured. Among the tested extract and fractions, the highest contents of total phenolic and flavonoidswere found in ethyl acetate fraction (117.8 mg GAE/g and 35.9 mg QE/g, respectively). Ethyl acetate fraction showed the highestDPPHandABTS radical scavenging activity, and the antioxidant activity of the other fractions followed the order n-hexane fraction>ethanol extract>methyl chloroform>n-butanol fraction. Inhibitory effect on the expression of matrix metalloproteinases (MMP1 and MMP3) was highest in the fraction of ethyl acetate, and n-butanol fraction also significantly inhibited the expression of MMP3. Antioxidant activities of L.
cuneata extracts were significantly positively related to their phenolic and flavonoid content.
CONCLUSION: Ethyl acetate fraction of L. cuneata ethanol extract showed potent antioxidant and matrix metalloproteinases inhibitory activities. Those activities might be related to the high total phenolic and flavonoid content of the extract.

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