Microponic system에서 배양액의 농도변화가감자 소식물체 생육에 미치는 영향 (The Effect of Nutrient Solution Concentration on Growth of Potato Plantlet in Microponic System)

서지정보

· 발행기관 : (사) 한국생물환경조절학회
· 수록지 정보 : 생물환경조절학회지 / 23권 / 2호 / 144 ~ 147페이지
· 저자명 : 고선아, 최기영, 이용범

초록

감자 가공용 신품종‘새봉’의 무병주 대량생산을 위하여microponic system에서 배양액의 농도변화가 감자 소식물체 생육에 미치는 영향을 구명하고자 수행 하였다. 조절한감자 배양액의 농도는 EC 0.2, 0.6, 1.0, 1.4, 1.8,14.0dS·m−1수준 이었으며 감자 조직배양묘는 생장점과 잎을 2개 포함한 1.5cm길이로 잘라 50mL 유리병(glass vial)에 1개씩 치상하여 18일, 또는 21일간 2회에 걸쳐 하였다.
배양액 량은 용기 당 2mL씩 넣고 10일 후 1 mL를 첨가하였으며, 환경조건은 일장 16시간, 온도 23 ± 1oC,40mmol−2·s−1의 백색 LED에서 실험 I의 배양액농도는 EC0.2, 1.0, 14dS·m−1였으며, 실험 II의 배양액농도는 0.6, 1.0,1.4, 1.8dS·m−1로 조정하였다. 치상 7일 후 소식물체의 생존율은 0.2dS·m−1에서 90%, 0.6dS·m−1에서 100%,1.0dS·m−1에서 100%, 1.4dS·m−1에서 0%, 1.8dS·m−1에서0%, 14.0dS·m−1에서 0% 이었다. 배양액의 전기전도도를1.0dS·m−1으로 조절한 처리에서 이식 2일 후 뿌리가 발육되어 소식물체 생육이 왕성하게 생장하여 18일 만에 7개의 엽, 길이 5cm, 생중 0.5g이상의 식물체로 생육하였다.
배양액의 농도를 0.6dS·m−1으로 조절한 처리에서는 이식4일 후부터 뿌리가 발육되어 21일 후 5개의 엽, 길이4cm, 생중 0.2g을 가진 식물체로 생육하였다. 따라서microponic system에서 무병 감자 묘 대량생산을 위해서는감자배양액의 전기전도도를 0.6~1.0dS·m−1 조절하여 관리하는 것이 효과적임을 알 수 있었다.

영어초록

It was intended to closely examine an effect that a change in the concentration of culture medium had on thepotato(Solanum tuberosum L.) plantlet growth in the microponic system so as to mass-produce the virus-free plant ofnew variety ‘Saebong’ for potato processing. The adjusted concentration of potato culture medium was 0.2, 0.6, 1.0, 1.4,1.8, and 14.0 dS· m−1. And potato seedling was cut into pieces of 1.5 cm in length, which included 2 growth points andleaves. And each was explanted in glass vial of 50 mL. And experiments were carried out twice for 18 days or 21days.
Culture medium of 2ml was put in the container respectively. And 1 mL was added after 10 days. And in terms of cultivationenvironment, the experiment was carried out at the day length of 16 hours at the temperature of 23 ± 1oC underthe white LED light of 40 μmol·m−2·s−1. The concentration of culture medium in the experiment I was EC 0.2, 1.0,14 dS·m−1 and was adjusted to 0.6, 1.0, 1.4, 1.8 dS·m−1 in the experiment II. The results showed that the survival rate ofplantlet was 90% at 0.2 dS ² m-1, 100% at 0.6 dS ² m-1, 100% at 1.0 dS ² m-1. 0% at 1.4 dS·m−1, 0% at 1.8 dS·m−1. and0% at 14.0 dS·m−1 after 7 days. With regard to the explanted potato seedling, in case of the treatment where the electricalconductivity of culture medium was adjusted to 1.0 dS·m−1, root developed 2 days after transplantation. And theplantlet vigorously grew into strong plant that had 7 leaves, length of 5cm, and fresh weight of 0.5 g after 18 days. Incase of the treatment where the concentration of culture medium was adjusted to 0.6 dS·m−1, the root plantlets developed4 days after transplantation. And those grew into plant that had 7 leaves and fresh weight of 0.2 g after 21 days.
Therefore, we found that it is effective to control potato culture medium by adjusting its electrical conductivity to0.6~1.0 dS·m−1 for the mass production of virus-free potato seedling in the microponic system.

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