The First Newborn Screening Study of T-Cell Receptor Excision Circle and к- Deleting Recombination Excision Circle for Severe Combined Immunodeficiency in Korea: A Pilot Study

서지정보

· 발행기관 : 대한소아감염학회
· 수록지 정보 : Pediatric Infection and Vaccine / 24권 / 3호 / 134 ~ 140페이지
· 저자명 : 손소희, 강지만, 김종민, 성세인, 김이슬, 이해정, 김빛아름, 이연경, 고선영, 신손문, 김예진

초록

Purpose: Severe combined immunodeficiency (SCID) is the most serious form of primary immunodeficiency. Infants with SCID are susceptible to life-threatening infections. To establish newborn screening for SCID in Korea, we performed a screening test for T-cell receptor excision circle (TREC) and к-deleting recombination excision circle (KREC) in neonates and investigated the awareness of SCID among their parents.
Methods: Collections of dried blood spots from neonates and parent surveys were performed at the Samsung Medical Center and Cheil General Hospital & Women's Healthcare Center in Korea. The amplification crossing point (Cp) value <37.0 was defined as TREC/KRECpositive based on cutoff values from measuring multiplex real-time polymerase chain reaction. A Cp value >39.0 was defined as negative.
Results: For TREC/KREC screening, 141 neonates were enrolled; 63 (44.7%) were male. One hundred forty neonates (99.3%) had positive TREC/KREC results at the time of the initial test; 82.3% and 75.9% were positive and 17.0% and 23.4% were weakly positive for TREC and KREC, respectively. In one neonate (0.7%), the initial TREC/KREC test result was negative. However, repeated tests obtained and confirmed a positive result. For an awareness survey, 168 parents were engaged. Only 2% of parents (3/168) knew that the newborn screening test for SCID had been introduced and performed in other countries. Eighty-four percent of parents (141/168) replied that nationwide newborn SCID screening should be performed in Korean newborns.
Conclusions: In this study, newborn SCID screening was performed along with assessment of public awareness of the SCID test in Korea.
The study results showed that newborn SCID screening can be readily applied for clinical use at a relatively low cost in Korea.

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