김치 추출 프로바이오틱스 섭취가 아토피 동물모델 NC/Nga mice에서면역 지표에 미치는 영향 (Effects of Probiotic Extracts of Kimchi on Immune Function in NC/Nga Mice)

서지정보

· 발행기관 : 한국식품과학회
· 수록지 정보 : 한국식품과학회지 / 40권 / 1호 / 82 ~ 87페이지
· 저자명 : 이인회, 이순희, 이인석, 박유경, 정대균, 조여원

초록

Atopic dermatitis (AD) is characterized by chronic relapsing inflammation and is associated with hyperproduction
of immunoglobulin E (IgE). Recent studies have suggested that one of the treatments to alleviate symptoms of
AD could be a supplementation of probiotics, Lactobacillus, Rhamnosus, Bifidus, etc. The purpose of this study was to
evaluate the effects of probiotics on immune parameters in NC/Nga mice treated with 1-chloro-2,4-dinitro-benzene
(DNCB). To induce atopic dermatitis, DNCB was treated to the back of mice for 2 weeks. Then, NC/Nga mice were
divided into the four experimental groups randomly. Probiotics fragment, probiotics with other complex (Lactobacillus
rhamnosus GG, Bifidobacterium lactis Bb-12LbL, L. plantarum K8, L. plantarum K8 fragment, γ-linolenic acid),
antihistamine, and distilled water were administrated orally to the NC/Nga mouse for 4 weeks of experimental period. The
groups were probiotics fragment group (DPF), probiotics with other complex group (DPOC), antihistamine group (DAH)
and distilled water group (DDW) as a control group. The levels of serum IgE, interlukin-4 (IL-4), interlukin-5 (IL-5),
interferon-gamma (IFN-γ) and spleenocyte IgE were measured. The levels of serum IgE were significantly different among
the four experimental groups. Before the treatment, there was no differences among the groups. However, from the first
through the third week of the treatments, the levels of serum IgE in the probiotics (DPF, DPOC) and antihistamine (DAH)
groups were lower than those of control group (p < 0.05). The levels of serum IL-4 of DPOC group was significantly
lower than that of control group (p < 0.05) and serum IL-5 levels of DPF, DPOC, and DAH groups were significantly
lower than that of control group. The levels of serum IFN-γ were not different among the four experimental groups. The
levels of serum IgE in supernatant of spleen lymphocytes were not significantly different among the groups. These results
suggest that probiotics supplementation showed partial effectiveness in the DNCB treated NC/Nga mice via modulation of
IgE level and IL-4, IL-5 production. Based on these findings, probiotics exhibited the inhibitory effect via IL-4 production
thereby inhibited the production of IgE in atopic animal model NC/Nga mice.

영어초록

Atopic dermatitis (AD) is characterized by chronic relapsing inflammation and is associated with hyperproduction
of immunoglobulin E (IgE). Recent studies have suggested that one of the treatments to alleviate symptoms of
AD could be a supplementation of probiotics, Lactobacillus, Rhamnosus, Bifidus, etc. The purpose of this study was to
evaluate the effects of probiotics on immune parameters in NC/Nga mice treated with 1-chloro-2,4-dinitro-benzene
(DNCB). To induce atopic dermatitis, DNCB was treated to the back of mice for 2 weeks. Then, NC/Nga mice were
divided into the four experimental groups randomly. Probiotics fragment, probiotics with other complex (Lactobacillus
rhamnosus GG, Bifidobacterium lactis Bb-12LbL, L. plantarum K8, L. plantarum K8 fragment, γ-linolenic acid),
antihistamine, and distilled water were administrated orally to the NC/Nga mouse for 4 weeks of experimental period. The
groups were probiotics fragment group (DPF), probiotics with other complex group (DPOC), antihistamine group (DAH)
and distilled water group (DDW) as a control group. The levels of serum IgE, interlukin-4 (IL-4), interlukin-5 (IL-5),
interferon-gamma (IFN-γ) and spleenocyte IgE were measured. The levels of serum IgE were significantly different among
the four experimental groups. Before the treatment, there was no differences among the groups. However, from the first
through the third week of the treatments, the levels of serum IgE in the probiotics (DPF, DPOC) and antihistamine (DAH)
groups were lower than those of control group (p < 0.05). The levels of serum IL-4 of DPOC group was significantly
lower than that of control group (p < 0.05) and serum IL-5 levels of DPF, DPOC, and DAH groups were significantly
lower than that of control group. The levels of serum IFN-γ were not different among the four experimental groups. The
levels of serum IgE in supernatant of spleen lymphocytes were not significantly different among the groups. These results
suggest that probiotics supplementation showed partial effectiveness in the DNCB treated NC/Nga mice via modulation of
IgE level and IL-4, IL-5 production. Based on these findings, probiotics exhibited the inhibitory effect via IL-4 production
thereby inhibited the production of IgE in atopic animal model NC/Nga mice.

참고자료

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