DNA 분석에 의한 팔색조의 암수 구분 및 암수별 피해 현황 그리고 크기 차이에 관한 연구 (The Study on the Sexual Difference in the Cause and the Time of Casualty and in the Size of the Fairy Pitta (Pitta nympha) through DNA Analysis in Republic of Korea)

서지정보

· 발행기관 : 한국환경과학회
· 수록지 정보 : 한국환경과학회지 / 23권 / 8호 / 1447 ~ 1453페이지
· 저자명 : 김은미, 전연선, 정길상, 김세재, 강창완, 오미래, 노푸름, 원현규

초록

The differentiation of sex is important for species preservation. However, Fairy Pitta is sexually monomorphic andsex of an individual is indistinguishable with its external characteristics. We determined the sex of Fairy Pitta throughDNA analysis and investigated the causes and time of injury and mortality and the size based on sex. We collected 21samples at Jeju Island, Korean Peninsula from 2004 to 2013 and extracted DNA from them and amplified chromohelicase DNA-binding gene from Z and W chromosomes through Polymerase Chain Reaction (PCR). We confirmedtheir sex with the banding pattern through Agarose gel electrophoresis, i.e. male (ZZ): one banded and female (ZW)two banded. We distinguished the sex of 17 of 21 samples resulting in 9 males and 8 females. Most casualties wererecorded in adult of both sexes. Causes of injury and mortality proved that female casualties occurred from windowstrikes, dehydration, car accident, predation by natural enemies, and male occurred from window strikes, car accidentand dehydration. The time of injury and mortality in adults differ by sex. There was no difference between sexes in anyof the six size parameters. As the time of injury and mortality differ by sex, the survey on the role and ecological natureby sex in breeding season must be carried out in the future. External measurements may not be reliable for sexing ofFairy Pitta and other traits such as vocal or characteristics are required to identify the sex of individuals in the field.

영어초록

The differentiation of sex is important for species preservation. However, Fairy Pitta is sexually monomorphic andsex of an individual is indistinguishable with its external characteristics. We determined the sex of Fairy Pitta throughDNA analysis and investigated the causes and time of injury and mortality and the size based on sex. We collected 21samples at Jeju Island, Korean Peninsula from 2004 to 2013 and extracted DNA from them and amplified chromohelicase DNA-binding gene from Z and W chromosomes through Polymerase Chain Reaction (PCR). We confirmedtheir sex with the banding pattern through Agarose gel electrophoresis, i.e. male (ZZ): one banded and female (ZW)two banded. We distinguished the sex of 17 of 21 samples resulting in 9 males and 8 females. Most casualties wererecorded in adult of both sexes. Causes of injury and mortality proved that female casualties occurred from windowstrikes, dehydration, car accident, predation by natural enemies, and male occurred from window strikes, car accidentand dehydration. The time of injury and mortality in adults differ by sex. There was no difference between sexes in anyof the six size parameters. As the time of injury and mortality differ by sex, the survey on the role and ecological natureby sex in breeding season must be carried out in the future. External measurements may not be reliable for sexing ofFairy Pitta and other traits such as vocal or characteristics are required to identify the sex of individuals in the field.

참고자료

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