저산소환경에 의한 송사리(Oryzias dancena)의 Stanniocalcin-2와 Hypoxia-Inducible Factor-1αmRNA 발현의 변화 (Changes in Stanniocalcin-2 and Hypoxia-Inducible Factor-1α mRNA Expression in Medaka Oryzias dancena Exposed to Acute Hypoxia)

서지정보

· 발행기관 : 한국수산과학회
· 수록지 정보 : 한국수산과학회지 / 46권 / 1호 / 70 ~ 76페이지
· 저자명 : 신지혜, 손영창

초록

Some fish live in aquatic environments with low or temporally changing O2 availability. Variation in dissolved oxygen (DO) levels requires behavioral, physiological, and biochemical adaptations to ensure the uptake of sufficient O2. Several species are relatively well adapted to tolerate low O2 partial pressures (hypoxia). The medaka (Oryzias dancena ) is an important model organism for biomedical research that shows remarkable tolerance to hypoxia. We investigated the regulation and role of hypoxia-inducible factor-1 (HIF-1α ) as a general hypoxia-response gene and stanniocalcin-2 (STC2 ), which is one of the genes regulated by HIF-1α in mammals under hypoxia. We subjected adult male medaka to the following three acute hypoxia regimes: 1, 24, and 72 h at DO = 1.8±0.5 ppm. The changes in STC2 and HIF-1α mRNA were monitored using quantitative real-time reverse-transcription PCR. We found strong upregulation of HIF-1α mRNA in the livers of fish exposed to hypoxia. Hypoxia rapidly upregulated STC-2 mRNA expression in muscle, but not in the brain, gills, liver, or intestine. Therefore, unlike in mammals, hypoxia might regulate O . dancena STC-2 expression in an HIF-1α-independent manner.

영어초록

Some fish live in aquatic environments with low or temporally changing O2 availability. Variation in dissolved oxygen (DO) levels requires behavioral, physiological, and biochemical adaptations to ensure the uptake of sufficient O2. Several species are relatively well adapted to tolerate low O2 partial pressures (hypoxia). The medaka (Oryzias dancena ) is an important model organism for biomedical research that shows remarkable tolerance to hypoxia. We investigated the regulation and role of hypoxia-inducible factor-1 (HIF-1α ) as a general hypoxia-response gene and stanniocalcin-2 (STC2 ), which is one of the genes regulated by HIF-1α in mammals under hypoxia. We subjected adult male medaka to the following three acute hypoxia regimes: 1, 24, and 72 h at DO = 1.8±0.5 ppm. The changes in STC2 and HIF-1α mRNA were monitored using quantitative real-time reverse-transcription PCR. We found strong upregulation of HIF-1α mRNA in the livers of fish exposed to hypoxia. Hypoxia rapidly upregulated STC-2 mRNA expression in muscle, but not in the brain, gills, liver, or intestine. Therefore, unlike in mammals, hypoxia might regulate O . dancena STC-2 expression in an HIF-1α-independent manner.

참고자료

· 없음