RAPD Marker를 이용한 Hedera속 식물의 다양성 조사 (Assessment of Genetic Diversity of Hedera spp. Using RAPD Marker Technique)

서지정보

· 발행기관 : 한국화훼학회
· 수록지 정보 : 화훼연구 / 16권 / 1호 / 28 ~ 35페이지
· 저자명 : 정미순, 최정근, 한태호, 정연화, 이자현, 김광수

초록

Hedera helix 11계통, Hedera rhombea 3계통, Fatshedera lizei 1계통, 그리고Fatsia japonica 1계통을수집하였다. RAPD primer 10 개를이용하여수집된 3속의재료들의유전적다양성을측정하였다. 3 속을재료로사용하여밴드간 96.9%의높은다형성을보였다. 총 97개의 RAPD 밴드를이진화하여 UPGMA 방법을이용하여계통도를작성하였다. Hedera helix 계통들은모두 1개의그룹에속하였으며 8계통의유전적거리는극도로적었으며나머지 3계통역시유전적으로가까웠다. 하지만형태적으로는높은다형성을보였다. 따라서수집된유전자원을이용한돌연변이체개발이가능성이있는방법으로제시되었다. Fatsia japonica 는유전적으로관계성이적어다른종들과평균 0.63의유전적거리를보였다. Hedera helix와Fatsia japonica 속간교배를통하여개발된Fatshedera lizei 는Hedera rhombea 계통들과함께계통도에서위치하였다.

영어초록

Eleven accessions of Hedera helix, three accessions of Hedera rhombea, one accession of Fat-shedera lizei, and one accession of Fatsia japonica were collected and their genetic diversity was measured by using 10 RAPD primers. Approximately ninety seven percentage of polymorphism was detected, because broad germplasm, three genus, was used. Total 97 bands were scored and a dendrogram was constructed by using an UPGMA method. Accessions belonging to Hedera helix tightly clustered in one group; eight acces­sions showed extremely narrow genetic differences and the other three accessions also showed significant sim­ilarity. Despite of their genetic similarity, they showed morphological variations. The morphological variation with a narrow genetic differences indicated that the ivy cultivars have been indeed developed from a mutation breeding program. Genetically most unrelated Fatsia japonica showed a genetic distance of 0.63 on the aver­age between other species. An accession from Fatshedera lizei developed by crossing between two genus, Hedera helix and Fatsia japonica, was allocated together with accessions from Hedera rhombea.

참고자료

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