중간엽 줄기세포의 연골세포로의 분화능에 (-)-에피갈로카테킨 갈레이트가 미치는 영향

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 6권 / 1호 / 243 ~ 249페이지
· 저자명 : 송지혜, 박부영, 김종일, 최성훈, 김동일

초록

The cell therapy using tissue-engineered cartilage is essential because the surgical operation for the repair of defected cartilage is not sufficient. Mesenchymal stem cells(MSCs) are an ideal candidate for use in cell-based
therapy because of their self-renewal ability and differentiation capability into specific cells such as chondrocytes. Especially, human umbilical cord blood(UCB) is an attractive cell source because it can be obtained easily and the
process is noninvasive and painless. However, the life span of MSCs are limited and the accumulation of intracellular oxidative damage by aging causes the loss of differentiation capacity during ex vivo expansion. (-)-Epigallocatechin gallate(EGCG) is known as an anti-oxidant agent which has a role as a free radical scavenger. It can improve long-term cell viability. Therefore, differentiation capacity can be maintained. In this study, effects of EGCG on chondrogenic potential of UCB-MSCs were investigated. The proliferation and differentiation capacity of UCBMSCs was characterized during expansion. Optimal concentration of EGCG was decided. Addition of EGCG in the media increased the cell viability during chondrogenic induction. Glycosaminoglycan(GAG), a cartilage-specific extracellular matrix(ECM), was also increased. It was confirmed that EGCG could improve the chondrogenic potential of UCB-MSCs by reducing oxidative stress. These results can contribute to the repair of damaged articular cartilage
using UCB-MSCs.

참고자료

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