듀얼 시린지펌프를 이용한 비손상 세포외 소포 분리법 (Non-Damaging Extracellular Vesicle Isolation Method Using a Dual Syringe Pump)

서지정보

· 발행기관 : 한국유체기계학회
· 수록지 정보 : 한국유체기계학회 논문집 / 28권 / 2호 / 110 ~ 115페이지
· 저자명 : 이재혁, 서보성, 홍정표, 이정원, 정인화

초록

The study investigates the filtration performance of a newly developed dual syringe pump, comparing it with a commercial peristaltic pump for micro-particle separation, such as extracellular vesicles (EVs). Using HCT116 cell culture medium, the peristaltic pump caused an increase in particle size distribution during extended circulation, indicating sample aggregation over time. This was reflected in significant increases in D10, D50, and D90 values, showing the broadening of particle sizes. In contrast, the dual syringe pump employs a two-stage mechanism, where one syringe pushes the sample while the other withdraws, effectively reducing pulsation. This design led to minimal changes in D10, D50, and D90 values even after extended recirculation, preserving sample integrity. Further validation involved a filtration process with commercial Spirulina for EV extraction. Transmission electron microscopy revealed that the dual syringe pump significantly reduced sample aggregation compared to the peristaltic pump, resulting in a more stable particle distribution. These findings highlight the dual syringe pump's benefits for long-term recirculation and filtration processes, providing a reliable solution for applications that require sample stability and reduced aggregation. The results indicate that the dual syringe pump effectively maintains the structural integrity of micro-particles during filtration, offering an improved approach for isolating EVs with less impact on particle size distribution, making it a valuable tool for researchers.

영어초록

The study investigates the filtration performance of a newly developed dual syringe pump, comparing it with a commercial peristaltic pump for micro-particle separation, such as extracellular vesicles (EVs). Using HCT116 cell culture medium, the peristaltic pump caused an increase in particle size distribution during extended circulation, indicating sample aggregation over time. This was reflected in significant increases in D10, D50, and D90 values, showing the broadening of particle sizes. In contrast, the dual syringe pump employs a two-stage mechanism, where one syringe pushes the sample while the other withdraws, effectively reducing pulsation. This design led to minimal changes in D10, D50, and D90 values even after extended recirculation, preserving sample integrity. Further validation involved a filtration process with commercial Spirulina for EV extraction. Transmission electron microscopy revealed that the dual syringe pump significantly reduced sample aggregation compared to the peristaltic pump, resulting in a more stable particle distribution. These findings highlight the dual syringe pump's benefits for long-term recirculation and filtration processes, providing a reliable solution for applications that require sample stability and reduced aggregation. The results indicate that the dual syringe pump effectively maintains the structural integrity of micro-particles during filtration, offering an improved approach for isolating EVs with less impact on particle size distribution, making it a valuable tool for researchers.

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