새로운 색지표를 이용한 우식활성검사법 개발 (Development of a new color scale for a caries activity test)

서지정보

· 발행기관 : 대한예방치과·구강보건학회
· 수록지 정보 : 대한구강보건학회지 / 34권 / 1호 / 9 ~ 17페이지
· 저자명 : 강시묵, 정회인, 정승화, 권호근, 김백일

초록

Objects: The aim of this study was to develop a new caries activity test based on colorimetric changes that could better reflect the amount of acid produced by oral bacteria as a broad spectrum of color than the previous test. The optimal candidates were combinations of pH indicators, and these were evaluated in pH buffering solutions and using dental plaque.
Materials and Methods: Six pH indicators,Bromocresol-purple (BCP), Bromocresol-green (BCG), Methyl-red (MR), Methyl-orange (MO), Resazurin (R), and Naphthyl-red (NR), were selected to show different colorsat various pH environments (range pH 3.0 ∼ 7.0). BCP and BCG are pH indicators that are already used in the Cariostat®, and they can show color changes from green to yellow. This study tried to broaden the color change spectrum from blue to red to give a more distinct differentiation at various pHs. Four pH indicators (MR, MO, R and NR) were blended with BCP and BCG. Each combination of the three indicators was assessed in different pH buffer systems: pH 7.0, 6.0, 5.0, 4.0, and 3.0. The selected combinations of pH indicators were applied to human dental plaque from 11 subjects (mean age: 27) to confirm the reproducibility of the in vitro results.
Results: According to the in vitro buffer system, the mixture of BCP, BCG and MR did not show any differences between pH 4.0 and pH 3.0. On the other hand, the mixtures of BCP-BCG-MO (ratio 2:1:1.5), BCP-BCG-R (1.5:1:0.5) and BCP-BCG-NR (2:1:1.5) showed distinguishable color changes from pH 7.0 to pH 3.0. Among the three candidates, the BCP-BCG-NR mixture showed the best color differences in the buffer solution and the human dental plaque cultivated solutions at various pHs.
Conclusion: This study evaluated a new colorimetric caries activity test that used a combination of several pH indicators. The new system can easily detect various pH environments from organic acid fermentation by using a wider range of colors (blue – dark green – green – orange – red).

영어초록

Objects: The aim of this study was to develop a new caries activity test based on colorimetric changes that could better reflect the amount of acid produced by oral bacteria as a broad spectrum of color than the previous test. The optimal candidates were combinations of pH indicators, and these were evaluated in pH buffering solutions and using dental plaque.
Materials and Methods: Six pH indicators,Bromocresol-purple (BCP), Bromocresol-green (BCG), Methyl-red (MR), Methyl-orange (MO), Resazurin (R), and Naphthyl-red (NR), were selected to show different colorsat various pH environments (range pH 3.0 ∼ 7.0). BCP and BCG are pH indicators that are already used in the Cariostat®, and they can show color changes from green to yellow. This study tried to broaden the color change spectrum from blue to red to give a more distinct differentiation at various pHs. Four pH indicators (MR, MO, R and NR) were blended with BCP and BCG. Each combination of the three indicators was assessed in different pH buffer systems: pH 7.0, 6.0, 5.0, 4.0, and 3.0. The selected combinations of pH indicators were applied to human dental plaque from 11 subjects (mean age: 27) to confirm the reproducibility of the in vitro results.
Results: According to the in vitro buffer system, the mixture of BCP, BCG and MR did not show any differences between pH 4.0 and pH 3.0. On the other hand, the mixtures of BCP-BCG-MO (ratio 2:1:1.5), BCP-BCG-R (1.5:1:0.5) and BCP-BCG-NR (2:1:1.5) showed distinguishable color changes from pH 7.0 to pH 3.0. Among the three candidates, the BCP-BCG-NR mixture showed the best color differences in the buffer solution and the human dental plaque cultivated solutions at various pHs.
Conclusion: This study evaluated a new colorimetric caries activity test that used a combination of several pH indicators. The new system can easily detect various pH environments from organic acid fermentation by using a wider range of colors (blue – dark green – green – orange – red).

참고자료

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