지방유래줄기세포가 부착된 콜라겐-히알루론산 지지체를 이용한 조직공학적 근육재생 (Hyaluronic Acid-Collagen Matrix Seeded with Adipose Derived Stem Cells as a Tissue-Engineering Approach to Rectus Abdominis Muscle Regeneration in Rats)

서지정보

· 발행기관 : 한국생체재료학회
· 수록지 정보 : 생체재료학회지 / 12권 / 3호 / 129 ~ 134페이지
· 저자명 : 심정수, 박세일, 문영미, 정규식, 정재호

초록

Adipose derived stem cells (ASCs) are multipotent cells capable of forming bone, cartilage, and other connective tissues.
We focused our attention on ASCs with myogenic potential which might be a useful function of stem cell-mediated
tissue engineering in various clinical situations related with muscular defects. ASCs could be easily harvested
from enzymatic digestion of human adipose tissue and readily culture expanded in vitro. Prior to seeding the cells
onto the porous hyaluronic acid-collagen matrix scaffolds of 8 mm diameter and 0.5mm thickness, the cells were
labeled with Feridex®. In 12 Sprague-Dawley rats, through mid-abdominal incisions, anterior sheath of rectus abdominis
muscle was opened and a circular muscle defect was made within rectus abdominis muscle. ASCs-seeded scaffold
discs were then placed within muscular defects in experimental group (n=6). In control group, scaffold discs
without stem cells were implanted into muscular defects (n=6). Six weeks after implantation, the recipients were sacrificed
and the tissue engineered constructs harvested. In Prussian blue stain, the specimens of experimental group
displayed numerous myoblasts and abundant blood vessels containing labeled Feridex® particles of dark blue color
within cytoplasm. In conclusion, the present results indicate that hyaluronic acid-collagen matrix scaffold is an effective
material for muscular and ASCs can be differentiated into myocyte within the scaffold and the cells also provoke
neovascularization. Further studies utilizing dynamic modalities in vitro and in vivo might be necessary for the refinement
of muscular tissue engineering.

영어초록

Adipose derived stem cells (ASCs) are multipotent cells capable of forming bone, cartilage, and other connective tissues.
We focused our attention on ASCs with myogenic potential which might be a useful function of stem cell-mediated
tissue engineering in various clinical situations related with muscular defects. ASCs could be easily harvested
from enzymatic digestion of human adipose tissue and readily culture expanded in vitro. Prior to seeding the cells
onto the porous hyaluronic acid-collagen matrix scaffolds of 8 mm diameter and 0.5mm thickness, the cells were
labeled with Feridex®. In 12 Sprague-Dawley rats, through mid-abdominal incisions, anterior sheath of rectus abdominis
muscle was opened and a circular muscle defect was made within rectus abdominis muscle. ASCs-seeded scaffold
discs were then placed within muscular defects in experimental group (n=6). In control group, scaffold discs
without stem cells were implanted into muscular defects (n=6). Six weeks after implantation, the recipients were sacrificed
and the tissue engineered constructs harvested. In Prussian blue stain, the specimens of experimental group
displayed numerous myoblasts and abundant blood vessels containing labeled Feridex® particles of dark blue color
within cytoplasm. In conclusion, the present results indicate that hyaluronic acid-collagen matrix scaffold is an effective
material for muscular and ASCs can be differentiated into myocyte within the scaffold and the cells also provoke
neovascularization. Further studies utilizing dynamic modalities in vitro and in vivo might be necessary for the refinement
of muscular tissue engineering.

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