Size-exclusion Chromatography를 활용한 가공식품 중 폴리아크릴산나트륨 분석법 확립 (Analytical Method for Sodium Polyacrylate in Processed Food Products by Using Size-exclusion Chromatography)

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 33권 / 6호 / 466 ~ 473페이지
· 저자명 : 정은정, 최유정, 이근영, 윤상순, 임호수, 김미경, 김용석

초록

An analytical method of sodium polyacrylate in processed food products was developed and monitored by using size-exclusion chromatography. GF-7M HQ column and UV/VIS detector were selected based on the peak shape and linearity. Flow rate, column oven temperature, and mobile phase were selected as 0.6 mL/min, 45℃, and 50 mM sodium phosphate buffer of pH 9.0, respectively. Samples for analysis of sodium polyacrylate were extracted with 50 mM sodium phosphate buffer of pH 7.0 for 3 hr at 20℃ and 150 rpm. Analytical method validation revealed proper selectivity and the calibration curve was selected in the range of 50∼500 mg/L, and the correlation coefficient of the calibration curve was more than 0.9985. Limit of detection of sodium polyacrylate was 10.95 mg/kg and the limit of quantification was 33.19 mg/kg. Accuracy and coefficient of variation for sodium polyacrylate analysis was 99.6∼127.6%, 3.0∼8.3% for intra-day and 94.3∼121.9%, 1.3∼2.6% for inter-day, respectively. Sodium polyacrylate was detected in 40 samples among monitored 125 processed food products. Detected contents were less than 0.2%, limited by the Food Additives Code. Results suggest the established size-exclusion chromatography method could be used to analyze the sodium polyacrylate in processed food products.

영어초록

An analytical method of sodium polyacrylate in processed food products was developed and monitored by using size-exclusion chromatography. GF-7M HQ column and UV/VIS detector were selected based on the peak shape and linearity. Flow rate, column oven temperature, and mobile phase were selected as 0.6 mL/min, 45℃, and 50 mM sodium phosphate buffer of pH 9.0, respectively. Samples for analysis of sodium polyacrylate were extracted with 50 mM sodium phosphate buffer of pH 7.0 for 3 hr at 20℃ and 150 rpm. Analytical method validation revealed proper selectivity and the calibration curve was selected in the range of 50∼500 mg/L, and the correlation coefficient of the calibration curve was more than 0.9985. Limit of detection of sodium polyacrylate was 10.95 mg/kg and the limit of quantification was 33.19 mg/kg. Accuracy and coefficient of variation for sodium polyacrylate analysis was 99.6∼127.6%, 3.0∼8.3% for intra-day and 94.3∼121.9%, 1.3∼2.6% for inter-day, respectively. Sodium polyacrylate was detected in 40 samples among monitored 125 processed food products. Detected contents were less than 0.2%, limited by the Food Additives Code. Results suggest the established size-exclusion chromatography method could be used to analyze the sodium polyacrylate in processed food products.

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