가교결합된 히알루론산 필름의 물리적/생물학적 평가 (Physical and Biological Evaluations of Cross-linked Hyaluronic Acid Film)

서지정보

· 발행기관 : 한국생체재료학회
· 수록지 정보 : 생체재료학회지 / 17권 / 4호 / 153 ~ 159페이지
· 저자명 : 박효승, 김아람, 노인섭

초록

Hyaluronic acid (HA) has been employed as a typical biomaterial in various medical areas such as dermal filler, scaffolds for tissue engineering and drug delivery, and etc. In this report, the HA films were fabricated by controlling the concentrations of 1,4-butanediol diglycidyl ether (BDDE) as a cross-linking agent, and their physical and biological properties have been evaluated. After swelling in water and dehydration by lyophilization of the cross-linked HA films, their morphologies and swelling behaviors after swelling in water for 24 hrs have been observed with scanning electron microscopy and measured with a microbalance, respectively. After homogenizing the films into microparticles, cell viability of the films was indirectly performed on the particles by covering the films with the HA films. Their cell compatibilities were evaluated by the assays of live & dead, thiazolyl blue tetrazolium bromide (MTT), bromodeoxyuridine (BrdU) and neutral red, by using extracts of the microparticles of HA films. The HA particles showed excellent cell compatibilities,showing possibilities of its applications as biomaterials.

영어초록

Hyaluronic acid (HA) has been employed as a typical biomaterial in various medical areas such as dermal filler, scaffolds for tissue engineering and drug delivery, and etc. In this report, the HA films were fabricated by controlling the concentrations of 1,4-butanediol diglycidyl ether (BDDE) as a cross-linking agent, and their physical and biological properties have been evaluated. After swelling in water and dehydration by lyophilization of the cross-linked HA films, their morphologies and swelling behaviors after swelling in water for 24 hrs have been observed with scanning electron microscopy and measured with a microbalance, respectively. After homogenizing the films into microparticles, cell viability of the films was indirectly performed on the particles by covering the films with the HA films. Their cell compatibilities were evaluated by the assays of live & dead, thiazolyl blue tetrazolium bromide (MTT), bromodeoxyuridine (BrdU) and neutral red, by using extracts of the microparticles of HA films. The HA particles showed excellent cell compatibilities,showing possibilities of its applications as biomaterials.

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