붉나무 캘러스 유도 및 그 추출물로부터 피부미백 효과에 관한 연구 (Induction and Proliferation of Callus in Rhus chinensis Mill. and Its Effect on Skin Whitening)

서지정보

· 발행기관 : 한국생약학회
· 수록지 정보 : 생약학회지 / 51권 / 4호 / 302 ~ 309페이지
· 저자명 : 김동명, 정주영, 이형곤, 권용성, 백진홍, 이관호, 장진훈, 한인석

초록

The objective of this study was to optimize the condition for induction and proliferation of callus from Rhus chinensis Mill. and investigate the skin-brightening effect of Rhus chinensis callus (RCC). It was confirmed that the most proper plant growth regulator (PGR) for callus induction is 1.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). The most optimal condition of PGR, medium and additives for callus proliferation were 2,4-D (1.0 mg/L), MS medium and citric acid, respectively. Inhibitory activities of tyrosinase were higher at 50 and 100 μg/mL of RCC extracts (41.86 and 75.56%, respectively) than arbutin (27.32%). As the results of measuring melanin inhibition in B16F1 melanocyte and B16F10 melanoma cell, RCC extracts increased its inhibitory activities concentration-dependently, and were found to have higher whitening effect than arbutin at a concentration of 100 μg/mL. Therefore, it is suggested that RCC can be used as an effective material for skin-brightening cosmetics.

영어초록

The objective of this study was to optimize the condition for induction and proliferation of callus from Rhus chinensis Mill. and investigate the skin-brightening effect of Rhus chinensis callus (RCC). It was confirmed that the most proper plant growth regulator (PGR) for callus induction is 1.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D). The most optimal condition of PGR, medium and additives for callus proliferation were 2,4-D (1.0 mg/L), MS medium and citric acid, respectively. Inhibitory activities of tyrosinase were higher at 50 and 100 μg/mL of RCC extracts (41.86 and 75.56%, respectively) than arbutin (27.32%). As the results of measuring melanin inhibition in B16F1 melanocyte and B16F10 melanoma cell, RCC extracts increased its inhibitory activities concentration-dependently, and were found to have higher whitening effect than arbutin at a concentration of 100 μg/mL. Therefore, it is suggested that RCC can be used as an effective material for skin-brightening cosmetics.

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