참굴 Crassostrea gigas 인공종묘생산 시 유생의 성장과 체성분 변화 (Larvae Growth and Biochemical Composition Change of the Pacific Oyster Crassostra gigas, Larvae during Artificial Seed Production)

서지정보

· 발행기관 : 한국양식학회
· 수록지 정보 : 한국양식학회지 / 21권 / 4호 / 203 ~ 212페이지
· 저자명 : 허영백, 민광식, 김태익, 이승주, 허성범

초록

A nutritional demand of oyster, Crassostrea gigas larva as part of research for improving of utilization of microalgae
being used for the artificial oyster seed production. The change of body growth and biochemical compositions
of larvae were investigated during larvae rearing in hatchery. The larvae were cultured in 60 M/T
tank and fed mixture 6 different phytoplankton species, Isochrysis galbana (30%), Cheatoceros gracilis
(20%), Pavlova lutheri (20%), Phaeodactylum triconutum (10%), Nannochryis oculata (10%) and
Tetraselmis tetrathele (10%). The initial feeding amount was 0.3x104cells/mL at three times a day to Dshaped
larva and the feeding amount had been increased 30% gradually every two day since the larvae were raising.
The larvae were developed from D shape to pediveliger stage for 12 days. The daily growth of shell length
and hight were 5.8~30.8 μm and 8.7~31.4 μm, respectively and weight gains were changed from D shape to pediveliger
as follow: wet weight was 0.52~15.0 μg/larva, dry weight was 0.2~6.5 μg/larva, and ash free dry weight
was 0.1~8.5 μg/larva. The larvae growth pattern shown a logarithmic phase from D shape to umbone stage but
after that stage shown a exponential growth aspect. The crude protein, crude lipid and nitrogen free extract (NFE)
of larvae during rearing periods were analyzed as 6.1~10.6%, 0.6~1.1% and 1.0-2.7%, respectively. And the
total amino acid contents of the larvae during rearing periods were in order as glutamic acid
1.26~2.24%, aspartic acid 0.97~1.70%, and methionine 0.12~0.33%. Of the total fatty acid in the analyzed larvae,
the saturated fatty acid (SSAFA) was decreased from 54.3% (D shaped larvae) to 17.1% (pediveliger) as larvae
development but the total mono-unsaturated fatty acid (ΣMOFA) and Poly-unsaturated fatty acid (ΣPUFA) were
increased from 29.9% and 7.8% to 40.6% and 45.6%, respectively. By the way the each fatty acid of the larvae
were composed of palmitic acid 9.89~36.95%, oleic acid 12.17~32.29%, linoleic acid 1.96~33.55%, EPA
2.17~11.58% and DHA 1.95~4.51%. As a result of this study, the larvae of oyster were demanded a various nutrients
for healthy growth and the feeding control, expecially after umbone stage larvae are a rapidly growing time,
is very important for success of artificial seed production.

영어초록

A nutritional demand of oyster, Crassostrea gigas larva as part of research for improving of utilization of microalgae
being used for the artificial oyster seed production. The change of body growth and biochemical compositions
of larvae were investigated during larvae rearing in hatchery. The larvae were cultured in 60 M/T
tank and fed mixture 6 different phytoplankton species, Isochrysis galbana (30%), Cheatoceros gracilis
(20%), Pavlova lutheri (20%), Phaeodactylum triconutum (10%), Nannochryis oculata (10%) and
Tetraselmis tetrathele (10%). The initial feeding amount was 0.3x104cells/mL at three times a day to Dshaped
larva and the feeding amount had been increased 30% gradually every two day since the larvae were raising.
The larvae were developed from D shape to pediveliger stage for 12 days. The daily growth of shell length
and hight were 5.8~30.8 μm and 8.7~31.4 μm, respectively and weight gains were changed from D shape to pediveliger
as follow: wet weight was 0.52~15.0 μg/larva, dry weight was 0.2~6.5 μg/larva, and ash free dry weight
was 0.1~8.5 μg/larva. The larvae growth pattern shown a logarithmic phase from D shape to umbone stage but
after that stage shown a exponential growth aspect. The crude protein, crude lipid and nitrogen free extract (NFE)
of larvae during rearing periods were analyzed as 6.1~10.6%, 0.6~1.1% and 1.0-2.7%, respectively. And the
total amino acid contents of the larvae during rearing periods were in order as glutamic acid
1.26~2.24%, aspartic acid 0.97~1.70%, and methionine 0.12~0.33%. Of the total fatty acid in the analyzed larvae,
the saturated fatty acid (SSAFA) was decreased from 54.3% (D shaped larvae) to 17.1% (pediveliger) as larvae
development but the total mono-unsaturated fatty acid (ΣMOFA) and Poly-unsaturated fatty acid (ΣPUFA) were
increased from 29.9% and 7.8% to 40.6% and 45.6%, respectively. By the way the each fatty acid of the larvae
were composed of palmitic acid 9.89~36.95%, oleic acid 12.17~32.29%, linoleic acid 1.96~33.55%, EPA
2.17~11.58% and DHA 1.95~4.51%. As a result of this study, the larvae of oyster were demanded a various nutrients
for healthy growth and the feeding control, expecially after umbone stage larvae are a rapidly growing time,
is very important for success of artificial seed production.

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