추출용매와 부위에 따른 고추 추출물의 세포독성 (Cytotoxicity of Extracts from Korean Pepper (Capsicum annuum L.) by Extraction Solvents and Plant Parts)

서지정보

· 발행기관 : 한국약용작물학회
· 수록지 정보 : 한국약용작물학회지 / 22권 / 5호 / 369 ~ 377페이지
· 저자명 : 최소라, 김명준, 안민실, 송은주, 서상영, 최민경, 김영선, 최동근, 송영주

초록

In order to find out anticancer activity of Korean pepper (Capsicum annuum L.), the cytotoxicity against 8cell lines including 293 (normal kidney cells) and A-431 (epidermoid carcinoma cells) of extracts by extraction solvents andplant parts were investigated using MTT assay. Also the correlation between content of capsaicin known as anticanceringredient and cytotoxicity of extracts from pepper were analyzed. The distilled water extracts from seed and germinatedseed showed very high cytotoxicity against 6 cancer cell lines including A549 (lung carcinoma cells), AGS (stomach adenocarcinomacells), HeLa (cervix adenocarcinoma cells), HepG2 (hepatoblastoma cells), HT-29 (colon adenocarcinoma cells),and MCF-7 (breast adenocarcinoma cells). But 80% ethanol and methanol extracts showed cytotoxicity against 293 andAGS. The RC50, that was, the concentration of sample required for 50% reduction of cell viability, of seed and germinatedseed extracts against AGS were 33.4 ~ 389.1㎍/㎖ and 63.9 ~ 1,316.7㎍/㎖, respectively, so anticancer activity was higher inseed than in germinated seed. In capsaicin contents, seed with high cytotoxicity and pericarp with a little cytotoxicity contained47.4 ~ 1,260.0㎍/g and 58.3 ~ 1,498.0㎍/g, respectively. As these results, the correlation was not between cytotoxicityand capsaicin content.

영어초록

In order to find out anticancer activity of Korean pepper (Capsicum annuum L.), the cytotoxicity against 8cell lines including 293 (normal kidney cells) and A-431 (epidermoid carcinoma cells) of extracts by extraction solvents andplant parts were investigated using MTT assay. Also the correlation between content of capsaicin known as anticanceringredient and cytotoxicity of extracts from pepper were analyzed. The distilled water extracts from seed and germinatedseed showed very high cytotoxicity against 6 cancer cell lines including A549 (lung carcinoma cells), AGS (stomach adenocarcinomacells), HeLa (cervix adenocarcinoma cells), HepG2 (hepatoblastoma cells), HT-29 (colon adenocarcinoma cells),and MCF-7 (breast adenocarcinoma cells). But 80% ethanol and methanol extracts showed cytotoxicity against 293 andAGS. The RC50, that was, the concentration of sample required for 50% reduction of cell viability, of seed and germinatedseed extracts against AGS were 33.4 ~ 389.1㎍/㎖ and 63.9 ~ 1,316.7㎍/㎖, respectively, so anticancer activity was higher inseed than in germinated seed. In capsaicin contents, seed with high cytotoxicity and pericarp with a little cytotoxicity contained47.4 ~ 1,260.0㎍/g and 58.3 ~ 1,498.0㎍/g, respectively. As these results, the correlation was not between cytotoxicityand capsaicin content.

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