고추탄저병 병원균(Colletotrichum coccodes)에 대한 배초향(Agastache rugosa)의 지상부로부터 항진균 활성물질 탐색 (Antifungal active substance from aerial parts of Agastache rugosa against the pepper anthracnose pathogen, Colletotrichum coccodes)

서지정보

· 발행기관 : 한국농약과학회
· 수록지 정보 : 농약과학회지 / 28권 / 3호 / 241 ~ 248페이지
· 저자명 : 박홍구, 전경미, 최용화

초록

고추탄저병의 원인균인 Colletotrichum coccodes에 대한 친환경 농자재 개발을 목적으로 배초향(Agastache Rugosa)의 지상부 추출물로부터 항진균 활성물질을 탐색하였다. 메탄올(MeOH)로 추출한 배초향의 지상부를 hexane, chloroform, ethyl acetate, butanol, aquous로 순차적으로 용매분획 하였고, 생물검정 결과 강한 활성을 보인 hexane fraction에서 silica gel column chromatography를 2회 반복하였다. 활성분획으로 분리한 구조는 (1R,4S,4aS,6R,8aS)-4,8a,9,9-tetramethyldecahydro –1,6-methanonaphthalen-1-ol(Patchouli alcohol)로 구조동정 되었다. 생물검정 결과, Patchouli alcohol의 C. coccodes에 대한 EC50값은 48.9ppm으로 측정되었으며 배초향 추출물은 식물 탄저병의 원인 진균 중 하나인 C. coccodes에 대해 항진균 활성을 가진 것으로 확인되었다. 따라서 Patchouli alcohol은 친환경 농업자재로 개발 활용이 가능할 것으로 기대된다.

영어초록

To develop an eco-friendly agent against Colletotrichum coccodes, the causative agent of anthracnose, including peppers, in peppers, an attempt was made to develop an antifungal agent using the aerial parts of Agastache rugosa. Based on the aerial parts of the Agastache rugosa extracted with methanol, solvent fractionation was sequentially attempted using solvents of hexane, chloroform, ethyl acetate, butanol, and water. Silica gel column chromatography was repeated twice on the hexane fraction, which showed the highest antifungal activity in the bioassay. The active structure was identified as (1R, 4S, 4aS, 6R, 8aS)-4, 8a, 9, 9-tetramethyldecahydro-1,6-methanonaphthalen-1-ol(Patchouli alcohol) by GC-MS. As a result of bioassay, the EC50 value for C. coccodes of Patchouli alcohol was measured to be 48.9 ppm. Accordingly, the extract of Agastache rugosa was confirmed to exhibit antifungal activity against C. coccodes, one of the causes of plant anthracnose. Therefore, Patchouli alcohol and is therefore expected to be used as an eco-friendly agricultural material.

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