Mesua ferrea L. 열매 유래 탄저병원균 Colletotrichum fructicola에 대한 항진균 활성물질 탐색 (Antifungal Activity Substances from Mesua ferrea L. Fruit Against Colletotrichum fructicola)

서지정보

· 발행기관 : 한국농약과학회
· 수록지 정보 : 농약과학회지 / 27권 / 3호 / 202 ~ 209페이지
· 저자명 : 전경미, 최용화

초록

탄저병 원인균인 Colletotrichum fructicola에 대한 친환경 농자재 개발을 목적으로 Mesua ferrea 열매의 추출물로부터 항진균 활성물질을 탐색하였다 . Mesua ferrea 열매의 MeOH 추출물을 hexane, chloroform, ethyl acetate, butanol, aquous로 순차적으로 용매분획 하였고 , 생물검정 결과 가장 강한 활성을 보인 hexane fraction을 silica gel column chromatography하여 활성물질을 분리 ·정제 하였다 . 최종 항진균 활성분획으로 분리 ·정제한 HSSC-6, HSSC- 8 subfraction을 GC-MS로 분석한 결과 -copaene과 kaur-16-ene으로 각각 구조동정 되었다 . -copaene의 EC50은73.4 ppm, kaur-16-ene의 EC50은 57.3 ppm으로 나타났으며 , kaur-16-ene의 활성이 -copaene 보다 다소 강한 활성을가진 것으로 확인이 되었다 . 따라서 , C. fructicola ICKb132에 대한 Mesua ferrea 열매의 추출물이 항진균 활성의 효과를 나타내었으므로 , 친환경 유기농자재로 개발이 가능할 것으로 판단되었다 .

영어초록

For the purpose of developing eco-friendly agricultural materials for Colletotrichum fructicola, the causative agent of anthracnose, antifungal active substances were explored from extracts of Mesua ferrea fruits. The methanol extract of the fruit of Mesua ferrea was sequentially subjected to solvent fractionation with hexane, chloroform, ethylacetate, butanol, and aqueous. As a result of the biological test, the active substance was separated and purified with silica gel column chromatography for hexane fraction, which showed the strongest activity. As a result of GC-MS analysis of HSSC-6 and HSSC-8 subfractions isolated and purified as final enhancement active fractions, the structures were identified as -copaene and kaur-16- ene, respectively. The EC50 of -copaene and kaur-16-ene were 73.4 ppm and 57.3 ppm, respectively. it was confirmed that the activity of kaur-16-ene was somewhat stronger than that of -copaene. Therefore, since the extract of Mesua ferrea fruit exhibited antifungal activity against C. fructicola ICKb132, the causative agent of anthracnose, it was judged to have the possibility of development as an eco-friendly agricultural material.

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