상추 균핵병 억제 및 생장촉진을 위한 길항 세균의 효능 (Efficacy of Antagonistic Bacterial Isolates in Suppressing Sclerotinia rot and Promoting Growth in Lettuce)

서지정보

· 발행기관 : 강원대학교 농업생명과학연구원
· 수록지 정보 : 농업생명환경연구 / 34권 / 3호 / 433 ~ 444페이지
· 저자명 : 김현승, 이윤수, 마헤시 아드히카리, 박효빈, 김기영, 이인규, 변은정, 우지민

초록

This study was conducted to biochemically characterize and screen the efficacy of antagonistic bacterial isolates in suppressing sclerotinia rot in lettuce crops. A total of 123 bacterial isolates were isolated from soil. In vitro antagonistic screening showed that 13 bacterial isolates were effective against Sclerotinia sclerotiorum and S. minor. In particular, two strains of Bacillus megaterium (HCS-1B and WJR-6B) showed the highest effectiveness against the tested pathogens, with a 75–80% inhibition rate. Additionally, the 13 bacterial isolates were biochemically characterized. Ammonia production, phosphate solubilization, protease production, starch hydrolysis and siderophore production, and HCN production were observed in 13, 3, 11, 9, and 2 bacterial isolates, respectively. Based on the results of the in vitro antagonistic test and biochemical characterization, the two best bacterial isolates (HCS-1B and WJR-6B) were selected for sclerotinia rot suppression and lettuce growth promotion under greenhouse conditions. The lowest incidence rate (0%) of sclerotinia rot was recorded in WJR-6B-treated lettuce pre-inoculated with S. sclerotiorum and in S. sclerotiorum-inoculated lettuce pretreated with HCS-1B. Combination treatment of HCS-1B and WJR-6B increased the leaf area of lettuce by approximately 3-fold (218.4 cm2) compared to the control (75 cm2). In this study, HCS-1B and WJR-6B were identified as potential antagonistic bacterial isolates that inhibit sclerotinia rot.
Field testing of these potential bacterial isolates would be a good direction for future studies.

영어초록

This study was conducted to biochemically characterize and screen the efficacy of antagonistic bacterial isolates in suppressing sclerotinia rot in lettuce crops. A total of 123 bacterial isolates were isolated from soil. In vitro antagonistic screening showed that 13 bacterial isolates were effective against Sclerotinia sclerotiorum and S. minor. In particular, two strains of Bacillus megaterium (HCS-1B and WJR-6B) showed the highest effectiveness against the tested pathogens, with a 75–80% inhibition rate. Additionally, the 13 bacterial isolates were biochemically characterized. Ammonia production, phosphate solubilization, protease production, starch hydrolysis and siderophore production, and HCN production were observed in 13, 3, 11, 9, and 2 bacterial isolates, respectively. Based on the results of the in vitro antagonistic test and biochemical characterization, the two best bacterial isolates (HCS-1B and WJR-6B) were selected for sclerotinia rot suppression and lettuce growth promotion under greenhouse conditions. The lowest incidence rate (0%) of sclerotinia rot was recorded in WJR-6B-treated lettuce pre-inoculated with S. sclerotiorum and in S. sclerotiorum-inoculated lettuce pretreated with HCS-1B. Combination treatment of HCS-1B and WJR-6B increased the leaf area of lettuce by approximately 3-fold (218.4 cm2) compared to the control (75 cm2). In this study, HCS-1B and WJR-6B were identified as potential antagonistic bacterial isolates that inhibit sclerotinia rot.
Field testing of these potential bacterial isolates would be a good direction for future studies.

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