가뭄저항성벼(CaMsrB2-8)의 환경위해성 평가 : 3세대의 후대안정성 연구 (Biosafety assessment for drought-tolerant transgenic rice (CaMsrB2-8) : Progeny stability study for three generations)

서지정보

· 발행기관 : 한국육종학회
· 수록지 정보 : 한국육종학회지 / 45권 / 2호 / 109 ~ 118페이지
· 저자명 : 오성덕, 류태훈, 이기종, 박순기, 박향미, 이시명, 안홍일, 손수인, 조현석

초록

The purpose of this research is a molecular characterization of introduced T-DNA in transgenic rice T4~T6 generation lines (#8) harboring a pepper MsrB2 gene under the control of stress inducible Rab21 promoter, as a part of biosafety evaluation for drought-tolerant transgenic rice (CaMsrB2-8). We identified the structure and sequence of transformation vector of T-DNA and analyzed insertion sites, flanking sequences, and generational stability of inserted T-DNA in transgenic rice. The transformation vector was consisted of right border, a drought-tolerant CaMsrB2-8 gene unit (Rab21 promoter::CaMsrB2::PinII terminator), a selectable marker herbicide resistance unit (CaMV 35S promoter::bar::Nos terminator), and left border in sequential order. We confirmed that T-DNA was introduced at the position of 41,737,284 bp of chromosome No. 1. based on the adaptor-ligation PCR and whole genome sequence database. T-DNAs were stably inherited through the T4 to T6 generations, and also stable expression of bar gene from T-DNA was confirmed. It was also confirmed that the backbone DNA of transformation vector containing antibacterial gene (aadA) was not present in CaMsrB2-8 rice genome. These results will be filed to biosafety assessment document of CaMsrB2-8 rice.

영어초록

The purpose of this research is a molecular characterization of introduced T-DNA in transgenic rice T4~T6 generation lines (#8) harboring a pepper MsrB2 gene under the control of stress inducible Rab21 promoter, as a part of biosafety evaluation for drought-tolerant transgenic rice (CaMsrB2-8). We identified the structure and sequence of transformation vector of T-DNA and analyzed insertion sites, flanking sequences, and generational stability of inserted T-DNA in transgenic rice. The transformation vector was consisted of right border, a drought-tolerant CaMsrB2-8 gene unit (Rab21 promoter::CaMsrB2::PinII terminator), a selectable marker herbicide resistance unit (CaMV 35S promoter::bar::Nos terminator), and left border in sequential order. We confirmed that T-DNA was introduced at the position of 41,737,284 bp of chromosome No. 1. based on the adaptor-ligation PCR and whole genome sequence database. T-DNAs were stably inherited through the T4 to T6 generations, and also stable expression of bar gene from T-DNA was confirmed. It was also confirmed that the backbone DNA of transformation vector containing antibacterial gene (aadA) was not present in CaMsrB2-8 rice genome. These results will be filed to biosafety assessment document of CaMsrB2-8 rice.

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