Metallothionein 유전자를 활용한 제주배꼽달팽이(Aegista chejuensis)의 분자계통학적 연구 (Molecular Phylogenetics of Korean endemic land snail, Aegista chejuensis inferred from Metallothionein gene sequence)

서지정보

· 발행기관 : 한국패류학회
· 수록지 정보 : The Korean Journal of Malacology / 34권 / 1호 / 59 ~ 65페이지
· 저자명 : 박지은, 조항철, 황희주, 정종민, 상민규, 민혜린, 강세원, 박소영, Bharat Bhusan Patnaik, 김완종, 한연수, 이준상, 이용석

초록

Metallothioneins (MT) are cysteine-rich small proteins known for their high affinity binding to metal ions. MT confers cytoprotection against oxidative stress and acts as an efficient modulator of immunity and infection. There have been ever-increasing reports on the function of MT in environmental monitoring programmes. Lately, MT has been studied as a biomarker towards the taxonomic clustering of various species, including the molluscans. The Korean endemic land snail, Aegista chejuensis is native to Jeju Island and South coastal regions of Korea. Functional genomics information for the species is limited, although the Illumina-based DNAseq and transcriptome information are registered with the Sequence Read Archive (SRA) of National Center for Biotechnology Information (NCBI). While annotating the ORF-sensitive unigenes from the transcriptome, a MT sequence comprising of 288 bp coding region and 96 amino acid residues translatable protein was deciphered. In silico analysis using ClustalX and the MEGA7 program grouped A. chejuensis under the Stylommatophora clade of eupulmonate mollusk families. The study was able to confirm the evolutionary position of the species and established the MT sequence as a candidate for taxonomic classification of the species.

영어초록

Metallothioneins (MT) are cysteine-rich small proteins known for their high affinity binding to metal ions. MT confers cytoprotection against oxidative stress and acts as an efficient modulator of immunity and infection. There have been ever-increasing reports on the function of MT in environmental monitoring programmes. Lately, MT has been studied as a biomarker towards the taxonomic clustering of various species, including the molluscans. The Korean endemic land snail, Aegista chejuensis is native to Jeju Island and South coastal regions of Korea. Functional genomics information for the species is limited, although the Illumina-based DNAseq and transcriptome information are registered with the Sequence Read Archive (SRA) of National Center for Biotechnology Information (NCBI). While annotating the ORF-sensitive unigenes from the transcriptome, a MT sequence comprising of 288 bp coding region and 96 amino acid residues translatable protein was deciphered. In silico analysis using ClustalX and the MEGA7 program grouped A. chejuensis under the Stylommatophora clade of eupulmonate mollusk families. The study was able to confirm the evolutionary position of the species and established the MT sequence as a candidate for taxonomic classification of the species.

참고자료

· 없음