포도 4배체 '후지미노리'와 3배체 '썸머블랙'의 교배로 얻은 미숙배의 기내배양 (In Vitro Culture of Immature Embryo Obtained by Crossing between Tetraploid Grape ‘Fujiminori’ and Triploid ‘Summer Black’)

서지정보

· 발행기관 : 한국원예학회
· 수록지 정보 : 원예과학기술지 / 31권 / 3호 / 352 ~ 358페이지
· 저자명 : 고재철, 오주은

초록

포도 4배체 ‘Fujiminori’에 3배체 ‘Summer Black’을 인공교배하여 얻은 미숙배의 발아 및 식물체 분화를 목적으로MS 배지에 GA3와 Coconut water(CW)를 농도별로 첨가하여 배배양을 실시하였다. ‘Fujiminori’에 ‘Summer Black’을교배하여 64.3%의 미숙배를 얻을 수 있었고, 미숙배를 기내배양한 결과 GA3 모든 처리구(0.01, 0.05, 0.25, 1.25mg･L-1)에서는 95% 이상의 발아율을 나타내었으나, 15.8-31.6%만이 정상적인 식물체로 생장하였다. 또한 GA3의 농도가 높아질수록 하배축은 신장되었고, 비대생장은 억제되었다. CW 모든 처리구(5, 10, 15, 20%) 중에 CW 10%와 CW 15% 처리구에서는 각각 68.4%와 66.7%로 많은 식물체를 획득할 수 있었다. 또한 줄기의 길이생장, 마디수, 발근수는 CW 10% 처리구에서, 뿌리길이는 CW 15% 처리구에서 양호한결과를 나타내었다. 따라서 포도 4배체 ‘Fujiminori’와 3배체 ‘Summer Black’의 인공교배를 통해 얻어진 미숙배의 발아 및 생장을 위해서는 CW 10-15%를 첨가한 MS 배지를이용한 배배양이 효과적이다.

영어초록

For the germination and differentiation of immature embryos obtained by artificial crossing between tetraploid grape ‘Fujiminori’ (Vitis vinifera × V. labruscana) and triploid ‘Summer Black’ (V. labruscana × V. vinifera), were incubated in vitro using MS medium supplemented with GA3 or coconut water (CW)at various concentrations. The percentage of embryo formation of ‘Fujiminori’ × ‘Summer Black’ was 64.3%.
Embryo germination percentage was higher than 95% in all the GA3 treatments at the concentrations of 0.01,0.05, 0.25, and 1.25 mg･L-1. However, only 15.8-31.6% of the germinated embryos successfully developed into normal plantlets. At higher concentration of GA3, the plantlets developed infirm hypocotyls with over elongated and less enlarged structure. Among the treatments of CW at the concentrations of 5, 10, 15, and 20% (v/v), 10% and 15% were more effective and plantlet achievement percentage were 68.4 and 66.7%,respectively. The addition of 10% CW was most effective to obtain plantlets with optimal shoot length,node and root numbers. 15% CW was suitable to obtain plantlets with longer roots. Accordingly, the embryo culture using the MS medium supplemented with 10-15% CW was observed to be more efficient for germinating and growing the immature embryos produced from artificial crossing between tetraploid grape ‘Fujiminori’and triploid ‘Summer Black’.

참고자료

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