RFLP, PLFA, CLSU를 이용한 폐기물연용토양의토양미생물 특성 평가 비교 (Comparison of Biological Characteristics on the Organic Waste-treated Lysimeter Soil by RFLP, PLFA, and CLSU)

서지정보

· 발행기관 : 한국토양비료학회
· 수록지 정보 : 한국토양비료학회지(Korean Journal of Soil Science and Fertilizer) / 41권 / 6호 / 415 ~ 418페이지
· 저자명 : 장갑열, 원항연, 이강효, 권순익, 공원식, 서장선, 성재모

초록

폐기물 연용토양의 미생물 군집 분석을 위해 공단지역 하수 슬러지 처리등 16처리(3수준)에 대하여 가장 좋은 방법을 찾고자, RFLP, CLSU, PLFA,TGGE 의 4가지 방법을 이용하였으며, 이들 4가지 방법에 대하여 시기별 군집변동 조사를 통한 통계적 비교 분석을 하였다. 그 결과, 미생물의 경시적 군집 변동상 관찰은 PLFAs (Phospholipid Fatty Acids) 방법이 가장 효율적이었으며 RFLP는 많은 종류의 제한 효소의 사용이 필요하다. TGGE는 DNA 추출을 통해비배양학적인 분석에서는 가장 좋은 방법이나 PCR조건 등 실험 과정에서 토양종류에따라 민감하게 반
응하여 비슷한 조성의 토양분석에 효율적이라고 생각한다.

영어초록

The application of sludge wastes into agricultural fields has been increasing annually in Korea. In particular, sewage sludge application has been widely accepted in decades. Sewage sludge application aid in the recycling of essential nutrients and act as a source of organic matter improving the structure and water-holding properties of the soil. The efficient use of sludge wastes, however, requires an individual assessment of waste products. This study assessed the biological characteristics of organic waste-treated
lysimeter soils and develop its indicator to assess the soil health of organic waste-treated lysimeter soils.
Several analytical techniques more recently developed such as restriction fragment length polymorphism (RFLP), phospholipid fatty acid (PLFA), and community level substrate utilization (CLSU) fingerprints allow for detailed analyses of soil microbial communities. PLFA and RFLP was, therefore, used in the study to characterize the microbial communities in soil without the need to isolate individual fungi and bacteria. PLFA, RFLP and CLSU have been utilized to assess microbial characteristics of the lysimeter soils with four different sludge wastes for eight consecutive years. Each of these methods was analyzed for a different aspect of soil microbial characteristics. The study would disclose those methods yielded highly reproductive results for each soil and allow distinguishing the soils based on the structures of specific geneand PLFA-pools more than CLSU fingerprints. PLFA methods, especially, revealed the same relative similarities of the treated soils based on cluster analysis of the biological characteristics. Pig manure
compost-treated soil, however, was only the same relative resemblance among the three methods. These results indicated that PLFA easily assessed the biological soil characterization.

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