축산물유래 Listeria monocytogenes의 virulence marker 및 gene 조사 (Exploration of Virulence Markers and Genes of Listeria monocytogenes Isolated from Animal Products)

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 23권 / 3호 / 248 ~ 256페이지
· 저자명 : 이철현, 송현호, 김미령, 강호조, 손원근

초록

To investigate the epidemiological characteristics of 68 Listeria monocytogenes isolates, including
11 reference strains and 57 isolates from imported US beef, domestic meats (beef, pork, chicken meat), raw milk,
and milk plants. L. monocytogenes was to evaluate the production of virulence proteins, such as hemolysin (LLO) and
lecithinase (LCP), the adsorption of Congo red (CRA), and to detect virulence genes using the polymerase chain reaction
(PCR). In the study of virulence protein production, 68(100%), 62(91.2%), and 54(79.4%) of the 68 L. monocytogenes
strains were positive for LLO production, the LCP test, and the CRA test, respectively, while strains of other
species, such as L. innocua, L. gray, L. murrayi, and L. welshimeri, were not. There were no significant differences
between L. monocytogenes serotypes and the ability to produce LLO or LCP. L. monocytogenesstrains had very high
hemolytic titers (2 to 16 fold), while the other Listeria species, other than L. ivanovii and L. seeligeri, did not. The
hemolysin activities of L. monocytogenes, L. ivanovii, and L. seeligeri usually exceeded 1.0 HU/mg, while those of
other Listeria spp. were less than 0.04 HU/mg. In the PCR assay, all of the L. monocytogenes strains contained the
hlyA, plcA, plcB, inlA, and inlB virulence genes and produced a product of the expected size. In the PCR of the actA
gene, the expected 385-bp product was seen in 39(57.4%) L. monocytogenesstrains, while an unexpected 268-bp
product was seen in 29(42.6%) strains. Most L. monocytogenes strains isolated from Hanwoo beef produced the 385-
bp actA gene product, while strains of imported US beef usually produced the 268-bp actA gene product. By contrast,
no virulence gene products were amplified in the other Listeria spp.

영어초록

To investigate the epidemiological characteristics of 68 Listeria monocytogenes isolates, including
11 reference strains and 57 isolates from imported US beef, domestic meats (beef, pork, chicken meat), raw milk,
and milk plants. L. monocytogenes was to evaluate the production of virulence proteins, such as hemolysin (LLO) and
lecithinase (LCP), the adsorption of Congo red (CRA), and to detect virulence genes using the polymerase chain reaction
(PCR). In the study of virulence protein production, 68(100%), 62(91.2%), and 54(79.4%) of the 68 L. monocytogenes
strains were positive for LLO production, the LCP test, and the CRA test, respectively, while strains of other
species, such as L. innocua, L. gray, L. murrayi, and L. welshimeri, were not. There were no significant differences
between L. monocytogenes serotypes and the ability to produce LLO or LCP. L. monocytogenesstrains had very high
hemolytic titers (2 to 16 fold), while the other Listeria species, other than L. ivanovii and L. seeligeri, did not. The
hemolysin activities of L. monocytogenes, L. ivanovii, and L. seeligeri usually exceeded 1.0 HU/mg, while those of
other Listeria spp. were less than 0.04 HU/mg. In the PCR assay, all of the L. monocytogenes strains contained the
hlyA, plcA, plcB, inlA, and inlB virulence genes and produced a product of the expected size. In the PCR of the actA
gene, the expected 385-bp product was seen in 39(57.4%) L. monocytogenesstrains, while an unexpected 268-bp
product was seen in 29(42.6%) strains. Most L. monocytogenes strains isolated from Hanwoo beef produced the 385-
bp actA gene product, while strains of imported US beef usually produced the 268-bp actA gene product. By contrast,
no virulence gene products were amplified in the other Listeria spp.

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