낙엽진흙버섯 자실체 유래 열수 추출물과 에탄올 침전물의 항바이러스활성 (Antiviral Activity of Hot-Water Extract and Its Ethanol Precipitate of Phellinus pini Fruiting Body)

서지정보

· 발행기관 : 한국미생물·생명공학회
· 수록지 정보 : 한국미생물·생명공학회지 / 37권 / 1호 / 33 ~ 41페이지
· 저자명 : 이새미, 김우중, 김현걸, 남재환, 신현동, 권두한, 박용일, 김성민, 이윤희, 나예슬

초록

The crushed fruiting body of Alaskan
Porodaedalea pini (Brot.) Murrill (syn. Phellinus pini) was extracted in boiling water for 4 h with the yield of 20.5% in dry mass. This hot-water extract showed significant antiviral activity by inhibiting the plaque formation
in HeLa cells by coxackievirus B3 (CVB3) and also showed highest inhibitory effect against neuraminidase activity among water extracts of various mushrooms. From the water extract, the ethanol precipitate (EP)
and supernatant fraction (ES) were obtained through 75% ethanol precipitation with the yield of 43.3% and 28.3 % in dry mass, respectively. Whereas ES did not show any detectable level of antiviral activity, EP
showed significant dose-dependent inhibition of plaque formation by CVB3 in HeLa cells with an EC50 (50%effective concentration) of 0.45 mg/mL. The cytotoxicity on HeLa cells by EP was relatively low with the
CC50 (50% cytotoxic concentration) of 2.25 mg/mL. EP also effectively inhibited neuraminidase activity in a dose-dependent manner showing up to 75% inhibition at 1.7 mg/mL. These results suggest that the hot-water
extract and its EP of P. pini fruiting body can be a candidate for the development of a potent broad-range antiviral agent against influenza virus(Flu) as well as CVB3. The major active component of EP was shown to be
a heteropolysaccharide-protein complex containing glucose as the main sugar residue with mole percentage of 79.8% and other sugars like galactose (19.2%), xylose (17%), mannose (5.8%), and fucose (4.6%) and a small
portion (12.7%, in mass) of protein.

영어초록

The crushed fruiting body of Alaskan
Porodaedalea pini (Brot.) Murrill (syn. Phellinus pini) was extracted in boiling water for 4 h with the yield of 20.5% in dry mass. This hot-water extract showed significant antiviral activity by inhibiting the plaque formation
in HeLa cells by coxackievirus B3 (CVB3) and also showed highest inhibitory effect against neuraminidase activity among water extracts of various mushrooms. From the water extract, the ethanol precipitate (EP)
and supernatant fraction (ES) were obtained through 75% ethanol precipitation with the yield of 43.3% and 28.3 % in dry mass, respectively. Whereas ES did not show any detectable level of antiviral activity, EP
showed significant dose-dependent inhibition of plaque formation by CVB3 in HeLa cells with an EC50 (50%effective concentration) of 0.45 mg/mL. The cytotoxicity on HeLa cells by EP was relatively low with the
CC50 (50% cytotoxic concentration) of 2.25 mg/mL. EP also effectively inhibited neuraminidase activity in a dose-dependent manner showing up to 75% inhibition at 1.7 mg/mL. These results suggest that the hot-water
extract and its EP of P. pini fruiting body can be a candidate for the development of a potent broad-range antiviral agent against influenza virus(Flu) as well as CVB3. The major active component of EP was shown to be
a heteropolysaccharide-protein complex containing glucose as the main sugar residue with mole percentage of 79.8% and other sugars like galactose (19.2%), xylose (17%), mannose (5.8%), and fucose (4.6%) and a small
portion (12.7%, in mass) of protein.

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