HPLC에 의한 차나무 잔가지(부산물)의 추출물 내 지표 성분의 정량분석 (Quantitative Determination of Marker Compounds in the Extracts of Camellia sinensis L. Sub-branches (Residual Products) by HPLC)

서지정보

· 발행기관 : 한국약용작물학회
· 수록지 정보 : 한국약용작물학회지 / 27권 / 1호 / 24 ~ 29페이지
· 저자명 : 이민성, 임현정, 정희석, 조해진, 우현심, 오유진, 이수인, 김현철, 안경환, 김영수, 김대욱

초록

Background: Camellia sinensis L.(CS) is a perennial evergreen species of plant whose leaves are used to produce tea. In this plant species, the parts used are the leaves, sub-branch parts are thrown out. Methods and Results: Ethanol extract of sub-branch parts was used for isolation of major compounds by column chromatography. Structures were identified as caffeine (1), (-)-epicatechin (2) and (-)-epicatechin gallate (3) by interpretation of spectroscopic analysis, including 1H- and 13C-NMR. High-performance liquid chromatography (HPLC) method was used to compare the quantitative level of marker compounds in various extraction solvents of sub-branch parts of CS. The content of caffeine, (-)-epicatechin, and (-)epicatechin gallate in 30% ethanol extract showed higher value with 3.28 ± 0.57 ㎎/g, 5.53 ± 0.88 ㎎/g, and 1.29 ± 0.24 ㎎/g, respectively. Conclusions: These results indicated that not only leaves parts but also sub-branch, could be a good source for the functional material and pharmaceutical industry.

영어초록

Background: Camellia sinensis L.(CS) is a perennial evergreen species of plant whose leaves are used to produce tea. In this plant species, the parts used are the leaves, sub-branch parts are thrown out. Methods and Results: Ethanol extract of sub-branch parts was used for isolation of major compounds by column chromatography. Structures were identified as caffeine (1), (-)-epicatechin (2) and (-)-epicatechin gallate (3) by interpretation of spectroscopic analysis, including 1H- and 13C-NMR. High-performance liquid chromatography (HPLC) method was used to compare the quantitative level of marker compounds in various extraction solvents of sub-branch parts of CS. The content of caffeine, (-)-epicatechin, and (-)epicatechin gallate in 30% ethanol extract showed higher value with 3.28 ± 0.57 ㎎/g, 5.53 ± 0.88 ㎎/g, and 1.29 ± 0.24 ㎎/g, respectively. Conclusions: These results indicated that not only leaves parts but also sub-branch, could be a good source for the functional material and pharmaceutical industry.

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