무 시들음병에 대한 간편한 대량 저항성 검정법 개발 (Development of an Efficient Mass-screening Method for Testing the Resistance of Radish to Fusarium Wilt)

서지정보

· 발행기관 : 한국식물병리학회
· 수록지 정보 : 식물병 연구 / 22권 / 3호 / 152 ~ 157페이지
· 저자명 : 이지현, 장경수, 최용호, 김헌, 최경자

초록

Root-dipping inoculation method has been used to investigate resistance of radish plants to Fusarium wilt. However, the method requires a lot of labor and time because of complicate procedure. This study was conducted to establish a simple and eḀective mass-screening method for resistant radish to Fusarium wilt. Radish seedlings of susceptible and resistant cultivars were used to investigate wounding method by scalpel, inoculum concentration, and pathogen-inoculated growth stage of seedlings. We established an efficient mass-screening method based on our results as following: Roots of 14-day-old seedlings of radish are cut with a scalpel at a 90o angle to a 2 cm-depth at a 1 cm-distance from main stem and then inoculated by pouring with a 10 ml-aliquot of a fungal spore suspension (1.0×107 conidia/ml) on soil. The inoculated plants are cultivated in a growth room at 25oC for about 4 weeks with 12-hour light a day. The proposed screening method enables to eḀectively select resistant from mass radish plants cultivars to Fusarium wilt.

영어초록

Root-dipping inoculation method has been used to investigate resistance of radish plants to Fusarium wilt. However, the method requires a lot of labor and time because of complicate procedure. This study was conducted to establish a simple and eḀective mass-screening method for resistant radish to Fusarium wilt. Radish seedlings of susceptible and resistant cultivars were used to investigate wounding method by scalpel, inoculum concentration, and pathogen-inoculated growth stage of seedlings. We established an efficient mass-screening method based on our results as following: Roots of 14-day-old seedlings of radish are cut with a scalpel at a 90o angle to a 2 cm-depth at a 1 cm-distance from main stem and then inoculated by pouring with a 10 ml-aliquot of a fungal spore suspension (1.0×107 conidia/ml) on soil. The inoculated plants are cultivated in a growth room at 25oC for about 4 weeks with 12-hour light a day. The proposed screening method enables to eḀectively select resistant from mass radish plants cultivars to Fusarium wilt.

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