글루코스산화효소와 금나노로드 입자의 다층막으로 구성된 촉매를 이용하여 측정한 글루코스 센싱에 대한 연구 (A Study on Glucose Sensing Measured by Catalyst Containing Multiple Layers of Glucose Oxidase and Gold Nano Rod)

서지정보

· 발행기관 : 한국수소및신에너지학회
· 수록지 정보 : 한국수소및신에너지학회논문집 / 26권 / 2호 / 179 ~ 183페이지
· 저자명 : 정용진, 현규환, 한상원, 민지홍, 천승규, 고원건, 권용재

초록

In this study, we propose a catalyst structure including enzyme and metal nano rod for glucose sensing.
In the catalyst structure, glucose oxidase (GOx) and gold nano rod (GNR) are alternatingly immobilized on the surface of carbon nanotube (CNT), while poly(ethyleneimine) (PEI) is inserted in between the GOx and GNR to fortify their bonding and give them opposite polarization ([GOx/GNR]nPEI/CNT). To investigate the impact of [GOx/GNR]nPEI/CNT on glucose sensing, some electrochemical measurements are carried out. Initially, their optimal layer is determined by using cyclic voltammogram and as a result of that, it is proved that [GOx/GNR/ PEI]2/CNT is the best layer. Its glucose sensitivity is 13.315 μAmM-1cm-2. When it comes to the redox reaction mechanism of flavin adenine dinucleotide (FAD) within [GOx/GNR/PEI]2/CNT, (i) oxygen plays a mediator role in moving electrons and protons generated by glucose oxidation reaction to those for the reduction reaction of FAD and (ii) glucose does not affect the redox reaction of FAD. It is also recognized that the [GOx/GNR/PEI]3/CNT is limited to the surface reaction and the reaction is quasi-reversible.

영어초록

In this study, we propose a catalyst structure including enzyme and metal nano rod for glucose sensing.
In the catalyst structure, glucose oxidase (GOx) and gold nano rod (GNR) are alternatingly immobilized on the surface of carbon nanotube (CNT), while poly(ethyleneimine) (PEI) is inserted in between the GOx and GNR to fortify their bonding and give them opposite polarization ([GOx/GNR]nPEI/CNT). To investigate the impact of [GOx/GNR]nPEI/CNT on glucose sensing, some electrochemical measurements are carried out. Initially, their optimal layer is determined by using cyclic voltammogram and as a result of that, it is proved that [GOx/GNR/ PEI]2/CNT is the best layer. Its glucose sensitivity is 13.315 μAmM-1cm-2. When it comes to the redox reaction mechanism of flavin adenine dinucleotide (FAD) within [GOx/GNR/PEI]2/CNT, (i) oxygen plays a mediator role in moving electrons and protons generated by glucose oxidation reaction to those for the reduction reaction of FAD and (ii) glucose does not affect the redox reaction of FAD. It is also recognized that the [GOx/GNR/PEI]3/CNT is limited to the surface reaction and the reaction is quasi-reversible.

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