초음파 처리 조건에 따른 집락형 유해남조류 Microcystis 세포수 변화 연구 (Colonial Cyanobacteria, Microcystis Cell Density Variations using Ultrasonic Treatment)

서지정보

· 발행기관 : 한국물환경학회
· 수록지 정보 : 한국물환경학회지 / 34권 / 2호 / 210 ~ 215페이지
· 저자명 : 이혜진, 박혜경, 허준, 이현제, 홍동균

초록

Colonial cyanobacteria, Microcystis cells are difficult to count since the thickness of colonies constrained by amorphous mucilage and makes it impossible to estimate the number of cells. Disaggregation of Microcystis colonies into single cell is needed to improve the accuracy and precision of cell density estimation collected from natural samples. Therefore ultrasonic treatment method was commonly used owing to the simplicity and immediacy of the procedure. However amplitude, frequency and duration of ultrasonic treatment also cause cell loss during the experiment and the optimal ultrasonic treatment were not standardized till now. The aim of this study was to investigate the optimal ultrasonic treatment by analyzing the cell density and the colony numbers tested in time period experiment. We collected colonial Microcystis from the Changnyeong-Haman weir area in the Nakdong River during the harmful algal boom periods from September to October in 2017. Ultrasonic treatment method was applied to disrupt the colonies into single cells to enumerate the cell density. Among the treatment conditions, the results from continuously treated for 100 seconds were found to be the optimum to reduce the colonies to a suspension of single cell without cell losses under high and low Microcystis cell density. Lugol iodine fixed cells followed by sonication showed less negative impact of cell damage within the optimal treatment time. Furthermore disaggregated cells treated by sonication enables microscopic observation more easily since gas vacuoles were collapsed to facilitate the sedimentation of the cells under the counting chamber for the quantitative enumeration of buoyant Microcystis cells.

영어초록

Colonial cyanobacteria, Microcystis cells are difficult to count since the thickness of colonies constrained by amorphous mucilage and makes it impossible to estimate the number of cells. Disaggregation of Microcystis colonies into single cell is needed to improve the accuracy and precision of cell density estimation collected from natural samples. Therefore ultrasonic treatment method was commonly used owing to the simplicity and immediacy of the procedure. However amplitude, frequency and duration of ultrasonic treatment also cause cell loss during the experiment and the optimal ultrasonic treatment were not standardized till now. The aim of this study was to investigate the optimal ultrasonic treatment by analyzing the cell density and the colony numbers tested in time period experiment. We collected colonial Microcystis from the Changnyeong-Haman weir area in the Nakdong River during the harmful algal boom periods from September to October in 2017. Ultrasonic treatment method was applied to disrupt the colonies into single cells to enumerate the cell density. Among the treatment conditions, the results from continuously treated for 100 seconds were found to be the optimum to reduce the colonies to a suspension of single cell without cell losses under high and low Microcystis cell density. Lugol iodine fixed cells followed by sonication showed less negative impact of cell damage within the optimal treatment time. Furthermore disaggregated cells treated by sonication enables microscopic observation more easily since gas vacuoles were collapsed to facilitate the sedimentation of the cells under the counting chamber for the quantitative enumeration of buoyant Microcystis cells.

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