담수 미세조류 Tetradesmus reginae의 세포 생장과 카로티노이드 생산량에 광파장이 미치는 영향 (Effect of Light Wavelength on Cell Growth and Carotenoid Production in Freshwater Green Microalga, Tetradesmus reginae)

서지정보

· 발행기관 : 한국생물공학회
· 수록지 정보 : KSBB Journal / 39권 / 4호 / 119 ~ 127페이지
· 저자명 : 홍선우, 홍성주, 지현종, 윤신영, 정민주, 이철균, 조복연, 김유호, 김진우, 남승원, 김지훈

초록

This study aimed to examine the effect of light wavelength on cell growth and carotenoid production in the freshwater green microalga Tetradesmus reginae during cultivation in bubble column photobioreactors (multi-cultivators) under different light colors (450 nm, blue; 660 nm, red; and CW5700K, white). The microalga cultured under white light showed the fastest specific cell growth rate (1.19 ± 0.00 /day) and highest fresh cell concentration (9.04 ± 0.01 g/L) on day 6. Next, the microalgal cells were cultivated under white light for 5 days to evaluate the effect of light wavelength on carotenoid production. Furthermore, the culture medium was replaced with an N- and P-depleted medium, and the microalgal cells were subjected to different light spectra to induce carotenoid production. Culture under blue light showed the highest carotenoid concentration (19.40 ± 0.19 mg/L) and the highest carotenoid productivity (2.08 ± 0.06 mg/L/day); however, cell concentration was the lowest (4.12 ± 0.00 mg/g dry cell weight) owing to the high carotenoid content. These results clearly show that effective growth and carotenoid production may be induced in Tetradesmus reginae at an appropriate light intensity and quality parameters. Thus, this study provides a basis for developing a light-supply strategy to increase microalgal biomass and carotenoid productivity.

영어초록

This study aimed to examine the effect of light wavelength on cell growth and carotenoid production in the freshwater green microalga Tetradesmus reginae during cultivation in bubble column photobioreactors (multi-cultivators) under different light colors (450 nm, blue; 660 nm, red; and CW5700K, white). The microalga cultured under white light showed the fastest specific cell growth rate (1.19 ± 0.00 /day) and highest fresh cell concentration (9.04 ± 0.01 g/L) on day 6. Next, the microalgal cells were cultivated under white light for 5 days to evaluate the effect of light wavelength on carotenoid production. Furthermore, the culture medium was replaced with an N- and P-depleted medium, and the microalgal cells were subjected to different light spectra to induce carotenoid production. Culture under blue light showed the highest carotenoid concentration (19.40 ± 0.19 mg/L) and the highest carotenoid productivity (2.08 ± 0.06 mg/L/day); however, cell concentration was the lowest (4.12 ± 0.00 mg/g dry cell weight) owing to the high carotenoid content. These results clearly show that effective growth and carotenoid production may be induced in Tetradesmus reginae at an appropriate light intensity and quality parameters. Thus, this study provides a basis for developing a light-supply strategy to increase microalgal biomass and carotenoid productivity.

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