인간 면역결핍 바이러스 양성환자의 항문부 첨규 콘딜로마에서 관찰되는 인유두종 바이러스의 아형에 관한 연구 (Detection and Typing of Human Papillomavirus in Anal Condyloma Acuminatum of HIV-positive Patients)

서지정보

· 발행기관 : 대한외과학회
· 수록지 정보 : Annals of Surgical Treatment and Research / 78권 / 2호 / 111 ~ 115페이지
· 저자명 : 문석배, 문상희, 박규주

초록

Purpose: Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (＋) Korean anal condyloma using PCR-based DNA microarray.
Methods: DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (＋) patients (M：F=15：2, mean age 35 years, 22∼59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip.
Results: Fourteen patients (9 HIV (＋), 5 HIV (−)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (＋) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (−) patients, 5 patients (55.6%) proved to have multiple infections in HIV (＋) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (＋) patients (44.5%) showed co-infection with high-risk HPV.
Conclusion: Multiple infection and co-infection with high-risk types are more prevalent in HIV (＋) condyloma patients compared to HIV (−) patients. HPV types on HIV (＋) male anal condyloma are similar to those detected in the Korean female uterine cervix.

영어초록

Purpose: Anal condyloma is an epithelial proliferative lesion caused by human papillomavirus (HPV) infection. The present study analyzed the HPV types detected in HIV (＋) Korean anal condyloma using PCR-based DNA microarray.
Methods: DNA was extracted from the condyloma tissue of 17 patients including 9 HIV (＋) patients (M：F=15：2, mean age 35 years, 22∼59 years). The 1st PCR was performed with a general primer on L1 region, and nested PCR on the products of the 1st PCR. PCR products were hybridized with a DNA chip.
Results: Fourteen patients (9 HIV (＋), 5 HIV (−)) showed positive HPV DNA. Overall, type 6 was the most common (N=11), and type 11 (N=6), type 53 (N=3) in order. Among HIV (＋) patients, type 6 was also the most common (N=7), then type 11 (N=5) and type 53 (N=3). In contrast to the HIV (−) patients, 5 patients (55.6%) proved to have multiple infections in HIV (＋) patients (2 double, 2 triple, 1 quadruple infection). Four of 9 HIV (＋) patients (44.5%) showed co-infection with high-risk HPV.
Conclusion: Multiple infection and co-infection with high-risk types are more prevalent in HIV (＋) condyloma patients compared to HIV (−) patients. HPV types on HIV (＋) male anal condyloma are similar to those detected in the Korean female uterine cervix.

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