안토시아닌의 항산화 활성 증진을 위한 후코이단 기반의 나노이온복합체 (Fucoidan Based Nanocomplex for Improving Antioxidant Activity of Anthocyanin)

서지정보

· 발행기관 : 한국생체재료학회
· 수록지 정보 : 생체재료학회지 / 16권 / 2호 / 60 ~ 66페이지
· 저자명 : 민대홍, 나건

초록

To improve the antioxidant efficiency of anthocyanin, fucoidan-anthocyanin nanocomplex has been investigated. The nanocomplex was fabricated by simple process in water phase to minimize loss of anthocyanin. In the nanocomplex,structure of anthocyanin was changed from flavylium cation to quinoidal base structure. Between 200 to 300 nm of spherical nanocomplex was observed by dynamic light scattering (DLS) and field emission scanning electron microscopy (FE-SEM). The nanocomplex has 10 fold lower critical assemble concentration (CAC) value (0.025 mg/ml) than free anthocyanin (0.25 mg/ml). The radical scavenging rate of anthocyanin against DPPH radicals and hydrogen peroxide were improved from 76% to 79% and from 20% to 60%, respectively. Finally, anticancer activity of anthocyanin was measured to estimate the in vitro radical scavenging activity. In the lipopolysaccharide (LPS) mediated reactive oxygen species (ROS) rich condition, the nanocomplex effectively reduced cancer cells viability (77%) to compared with free anthocyanin (121%). Therefore, we conclude that fucoidan-anthocyanin nanocomplex has a potential for use as a carrier for anthocyanin delivery. These nanocomplexes would prove useful application in the food, beverage and pharmaceutical industries.

영어초록

To improve the antioxidant efficiency of anthocyanin, fucoidan-anthocyanin nanocomplex has been investigated. The nanocomplex was fabricated by simple process in water phase to minimize loss of anthocyanin. In the nanocomplex,structure of anthocyanin was changed from flavylium cation to quinoidal base structure. Between 200 to 300 nm of spherical nanocomplex was observed by dynamic light scattering (DLS) and field emission scanning electron microscopy (FE-SEM). The nanocomplex has 10 fold lower critical assemble concentration (CAC) value (0.025 mg/ml) than free anthocyanin (0.25 mg/ml). The radical scavenging rate of anthocyanin against DPPH radicals and hydrogen peroxide were improved from 76% to 79% and from 20% to 60%, respectively. Finally, anticancer activity of anthocyanin was measured to estimate the in vitro radical scavenging activity. In the lipopolysaccharide (LPS) mediated reactive oxygen species (ROS) rich condition, the nanocomplex effectively reduced cancer cells viability (77%) to compared with free anthocyanin (121%). Therefore, we conclude that fucoidan-anthocyanin nanocomplex has a potential for use as a carrier for anthocyanin delivery. These nanocomplexes would prove useful application in the food, beverage and pharmaceutical industries.

참고자료

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