세포 호흡량 측정용 다채널 측정 시스템 개발 (Development of a multi channel measurement system for the cellular respiration measurement)

서지정보

· 발행기관 : 한국센서학회
· 수록지 정보 : 센서학회지 / 19권 / 1호 / 36 ~ 42페이지
· 저자명 : 남현욱, 박정일, 김영미, 박정호

초록

This paper describes a multi channel measurement system which can measure the cellular respiration level in a solution containing cells by using a Clark-type sensor with the solution temperature control unit. The Clark-type sensor can measure the cellular respiration level in the solution because it can measure the reduction current depending on the dissolved oxygen level in the solution. This measurement system was maintained the temperature within ± 0.1 oC of the setting temperature value by on/off control method in order to measure the precise cellular respiration level. The measurement system showed that the applied voltage to the working electrode was very stable(−0.8 V± 0.0071 V) by using proportional control method.
From the current measurement, the response time and the linearity correlation coefficient were 25 sec and 0.94, respectively, which are very close to the results of the commercial product. Using this system and the fabricated Clarktype sensor, the average ratio of the uncoupled OCR(oxygen consumption rate) to the coupled OCR was 1.35 and this is almost the same as that obtained from a commercial systems.

영어초록

This paper describes a multi channel measurement system which can measure the cellular respiration level in a solution containing cells by using a Clark-type sensor with the solution temperature control unit. The Clark-type sensor can measure the cellular respiration level in the solution because it can measure the reduction current depending on the dissolved oxygen level in the solution. This measurement system was maintained the temperature within ± 0.1 oC of the setting temperature value by on/off control method in order to measure the precise cellular respiration level. The measurement system showed that the applied voltage to the working electrode was very stable(−0.8 V± 0.0071 V) by using proportional control method.
From the current measurement, the response time and the linearity correlation coefficient were 25 sec and 0.94, respectively, which are very close to the results of the commercial product. Using this system and the fabricated Clarktype sensor, the average ratio of the uncoupled OCR(oxygen consumption rate) to the coupled OCR was 1.35 and this is almost the same as that obtained from a commercial systems.

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