원유에서 분리한 Lactobacillus zeae RMK354의 생리적 특성 및 ACE 억제능 (Physiological Characteristics and ACE Inhibitory Activity of Lactobacillus zeae RMK354 Isolated from Raw Milk)

서지정보

· 발행기관 : 한국축산식품학회
· 수록지 정보 : 한국축산식품학회지 / 28권 / 5호 / 587 ~ 595페이지
· 저자명 : 임상동, 김기성, 도정룡

초록

In order to develop a new starter for fermented milk, 1037 bacterial strains were isolated from raw milk. The strain that
showed excellent acid producing and angiotensin converting enzyme (ACE) inhibitory activity (88.6%) was selected and
identified as a Lactobacillus zeae based on the result of API carbohydrate fermentation pattern and 16S rDNA sequence.
Lactobacillus zeae RMK354 was investigated further to study its physiological characteristics. It showed strong ACE inhibitory
activity compared with commercial LAB starters tested. The optimum growth temperature of L. zeae RMK354 was
40oC and it took 10 hr to reach pH 4.3 under this condition. L. zeae RMK354 showed more sensitive to penicillin-G, bacitracin,
novobiocin, in a comparison of 14 different antibiotics, and showed most resistance to polymyxin B and vancomycin.
It showed higher esterase and leucine arylamidase activities compared with 16 other enzymes. It was comparatively tolerant
to bile juice and able to survive at pH 2 for 3 hr. It showed inhibitory activity against Salmonella Typhimurium with the rate
of 60%. Based on these and previous results, L. zeae RMK354 could be an excellent starter culture for fermented milk with
high level of ACE inhibitory activity.

영어초록

In order to develop a new starter for fermented milk, 1037 bacterial strains were isolated from raw milk. The strain that
showed excellent acid producing and angiotensin converting enzyme (ACE) inhibitory activity (88.6%) was selected and
identified as a Lactobacillus zeae based on the result of API carbohydrate fermentation pattern and 16S rDNA sequence.
Lactobacillus zeae RMK354 was investigated further to study its physiological characteristics. It showed strong ACE inhibitory
activity compared with commercial LAB starters tested. The optimum growth temperature of L. zeae RMK354 was
40oC and it took 10 hr to reach pH 4.3 under this condition. L. zeae RMK354 showed more sensitive to penicillin-G, bacitracin,
novobiocin, in a comparison of 14 different antibiotics, and showed most resistance to polymyxin B and vancomycin.
It showed higher esterase and leucine arylamidase activities compared with 16 other enzymes. It was comparatively tolerant
to bile juice and able to survive at pH 2 for 3 hr. It showed inhibitory activity against Salmonella Typhimurium with the rate
of 60%. Based on these and previous results, L. zeae RMK354 could be an excellent starter culture for fermented milk with
high level of ACE inhibitory activity.

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