세포원심분리기를 사용한 뇌척수액산정검사의 세포회수율 (Cell Yield of Cerebrospinal Fluid Cell Count Using Cytocentrifuges)

서지정보

· 발행기관 : 대한임상검사과학회
· 수록지 정보 : 대한임상검사과학회지 / 45권 / 1호 / 37 ~ 42페이지
· 저자명 : 구본경, 심현설, 오정아, 이용탁, 최대용, 이범세, 김은지, 이승태, 김선희

초록

The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of 0∼5 WBCs/μL of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of 0∼5 WBCs/μL on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0∼40 cells in the 44 samples of 0 WBC/μL, and 3∼95 cells in the 31 samples of 1 WBC/μL. It was observed that the nucleated cell number for cells recovered on slide was 13∼100 cells in the 44 samples of 2 WBCs/μL, and more than 100 cells in the 29 samples of 3∼5 WBCs/μL, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of 0∼5 WBCs/μL. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as 1 WBC/μL in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at 0 WBC/μL by using the cell yield in a comparison between the value of 0∼5 WBCs/μL and nucleated cell number for cells recovered on slide.

영어초록

The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of 0∼5 WBCs/μL of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of 0∼5 WBCs/μL on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0∼40 cells in the 44 samples of 0 WBC/μL, and 3∼95 cells in the 31 samples of 1 WBC/μL. It was observed that the nucleated cell number for cells recovered on slide was 13∼100 cells in the 44 samples of 2 WBCs/μL, and more than 100 cells in the 29 samples of 3∼5 WBCs/μL, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of 0∼5 WBCs/μL. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as 1 WBC/μL in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at 0 WBC/μL by using the cell yield in a comparison between the value of 0∼5 WBCs/μL and nucleated cell number for cells recovered on slide.

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