감마선 조사에 의한 헤파린이 도입된 박테리아 셀룰로오스의 골조직공학용 지지체로서의 개발 및 특성분석 (Development and Characterization of Heparin Immobilized Bacterial Cellulose(BC) for bone Tissue Engineering using Gamma-irradiation)

서지정보

· 발행기관 : 한국조직공학과 재생의학회
· 수록지 정보 : 조직공학과 재생의학 / 11권 / 4호 / 56 ~ 63페이지
· 저자명 : 정성인, 정진오, 최종배, 신영민, 박종석, 권희정, 노영창, 안성준, 박만용, 임윤묵

초록

Bacterial cellulose(BC) scaffolds are generated from gel by gluconacetobacter hansenii TL-2C. BC hasgood properties such as high-burst pressure and the ultrafine highly nanofibrous structure of mimic natural extra celluarmatrix(ECM) for tissue engineering. In this study, 2-aminoethyl methacrylate(AEMA) was grafted onto BC surfaceusing gamma-irradiation and then heparin immobilized it using EDC/NHS reaction. The surface morphologyof scaffolds were confirmed BC membranes by SEM for heparin and AEMA grafted it, so that it was not changedsurface morphology. The result of TBO, fluorescamine staining, ATR-FTIR confirmed that heparin and AEMAimmobilized it. Also result of in vitro test, heparin immobilized AEMA-BC proliferation of hMSC to higher thanunmodified BC by CCK-8 and ALP activity then heparin immobilized AEMA-BC cumulative of BMP-2 was confirmeddecrease by release test. Therefore, heparin and AEMA grafted BC scaffolds have been possible to tissuescaffold in bone tissue engineering.

영어초록

Bacterial cellulose(BC) scaffolds are generated from gel by gluconacetobacter hansenii TL-2C. BC hasgood properties such as high-burst pressure and the ultrafine highly nanofibrous structure of mimic natural extra celluarmatrix(ECM) for tissue engineering. In this study, 2-aminoethyl methacrylate(AEMA) was grafted onto BC surfaceusing gamma-irradiation and then heparin immobilized it using EDC/NHS reaction. The surface morphologyof scaffolds were confirmed BC membranes by SEM for heparin and AEMA grafted it, so that it was not changedsurface morphology. The result of TBO, fluorescamine staining, ATR-FTIR confirmed that heparin and AEMAimmobilized it. Also result of in vitro test, heparin immobilized AEMA-BC proliferation of hMSC to higher thanunmodified BC by CCK-8 and ALP activity then heparin immobilized AEMA-BC cumulative of BMP-2 was confirmeddecrease by release test. Therefore, heparin and AEMA grafted BC scaffolds have been possible to tissuescaffold in bone tissue engineering.

참고자료

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