당뇨쥐 상악골에서 즉시 임플란트 후 골유착에 대한 면역조직학적 연구 (Immunohistochemical Study of Bone Response to the Immediate Implantation in the Rat Maxilla)

서지정보

· 발행기관 : 대한통합치과학회
· 수록지 정보 : 대한통합치과학회지 / 6권 / 2호 / 39 ~ 45페이지
· 저자명 : 표성운, 박수현, 조창준, 신현정, 허현아, 최재호

초록

Purpose: Dental implants are used successfully for restoration of dental function. However, the impact of diabetes
on the healing of bone associated with immediately placed dental implants is not completely understood. And little
is known about the expression of biological molecules involved in bone healing around implants. The purpose
of this study was to investigate the bone response around immediately placed titanium implants in the maxilla of
ovariectomized rats by immunohistochemical method.
Materials and methods: 36 rats were divided into the control, insulin-treated and diabetic groups. Rats received
streptozotocin (60 mg/kg) to induce diabetes; animals in the insulin-treated group also received 3 units of subcutaneous
slow-release insulin. Two titanium implants (1.2×3 mm) were placed in the extraction socket of maxillary first molars
of animals and specimens were harvested at 3 days, 1, 2 and 4 weeks. The expression levels of bone morphogenetic
protein (BMP)-4, transforming growth factor (TGF)-β and osteonectin (ON) were measured.
Result: The BMP-4 expression increased immediately all groups at day 3, week 1 and week 2. However, staining
intensity of diabetic group was weaker than the control and the insulin-treated group but not significant. At week 4,
in all groups, the mount of BMP-4 expression decreased. The TGF-β expression instantly showed at day 3 in control
group only. At week 1 and 2, TGF-β staining gradually increased in all group. But in the all group staining intensity
decreased at 4 week. And we found the expression of ON in the control and the insulin-treated group were higher than
that of the diabetic group at 1 weeks and 4 weeks, indicated that bone formation be active in those groups.
Conclusion: In this study, insulin treatment increased expression of biologic molecules in the bone around the
immediate placed titanium implants in the maxilla of diabetic rat. It is expected that insulin might reverse the reduced
predictability of success of immediate implantation with the uncontrolled diabetes patients.

영어초록

Purpose: Dental implants are used successfully for restoration of dental function. However, the impact of diabetes
on the healing of bone associated with immediately placed dental implants is not completely understood. And little
is known about the expression of biological molecules involved in bone healing around implants. The purpose
of this study was to investigate the bone response around immediately placed titanium implants in the maxilla of
ovariectomized rats by immunohistochemical method.
Materials and methods: 36 rats were divided into the control, insulin-treated and diabetic groups. Rats received
streptozotocin (60 mg/kg) to induce diabetes; animals in the insulin-treated group also received 3 units of subcutaneous
slow-release insulin. Two titanium implants (1.2×3 mm) were placed in the extraction socket of maxillary first molars
of animals and specimens were harvested at 3 days, 1, 2 and 4 weeks. The expression levels of bone morphogenetic
protein (BMP)-4, transforming growth factor (TGF)-β and osteonectin (ON) were measured.
Result: The BMP-4 expression increased immediately all groups at day 3, week 1 and week 2. However, staining
intensity of diabetic group was weaker than the control and the insulin-treated group but not significant. At week 4,
in all groups, the mount of BMP-4 expression decreased. The TGF-β expression instantly showed at day 3 in control
group only. At week 1 and 2, TGF-β staining gradually increased in all group. But in the all group staining intensity
decreased at 4 week. And we found the expression of ON in the control and the insulin-treated group were higher than
that of the diabetic group at 1 weeks and 4 weeks, indicated that bone formation be active in those groups.
Conclusion: In this study, insulin treatment increased expression of biologic molecules in the bone around the
immediate placed titanium implants in the maxilla of diabetic rat. It is expected that insulin might reverse the reduced
predictability of success of immediate implantation with the uncontrolled diabetes patients.

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