Carbapenem Inactivation Method: Accurate Detection and Easy Interpretation of Carbapenemase Production in Enterobacteriaceae and Pseudomonas spp.

서지정보

· 발행기관 : 대한임상미생물학회
· 수록지 정보 : Annals of Clinical Microbiology / 19권 / 4호 / 83 ~ 87페이지
· 저자명 : 송원근, 김한성, 김재석, 김현수, 신동훈, 신새암, 박민정

초록

Background: We evaluated the carbapenem inactivation method (CIM) compared with the modified Hodge test (MHT) for the detection of carbapenemase- producing Gram-negative bacilli.
Methods: A total of 61 isolates of carbapenemaseproducing Enterobacteriaceae (CPE: 14 KPC, 7 GES- 5, 8 NDM-1, 9 VIM-2, 9 IMP-1, and 14 OXA-48-like), 34 isolates of metallo-β-lactamase (MBL)-producing Pseudomonas spp. (14 VIM-2 and 20 IMP-6), and 70 carbapenem-nonsusceptible carbapenemase-negative isolates were included. The CIM and MHT were performed for all of the isolates. To perform the CIM, a meropenem disk was incubated with a suspension of the isolate to be tested and then on Mueller-Hinton agar with the Escherichia coli ATCC 29522 strains. The absence of an inhibition zone indicates presence of a carbapenemase. The presence of a clearing zone indicates lack of a carbapenemase.
Results: The total sensitivity and specificity of CIM (96% sensitivity and 100% specificity) in carbapenemnonsusceptible Enterobacteriaceae and Pseudomonas spp. were better than those of the MHT (77% sensitivity and 94% specificity). The interpretation of CIM results was easy, with no or ＜20 mm inhibition zones indicating positivity and ＞20 mm inhibition zones indicating negative carbapenemase activity.
Conclusion: The CIM had excellent sensitivity and specificity for detection of CPE and MBL-producing Pseudomonas spp., and a positive result was easily determined, unlike the MHT.

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