쥐의 경동맥 손상 모형에서 고용량 체외 방사선 조사가 Matrix Metalloprotease-2 발현에 미치는 영향 (Effect of High Dose External Irradiation on the Matrix Metalloprotease-2 Expression in a Rat Carotid Artery Injury Model)

서지정보

· 발행기관 : 대한심장학회
· 수록지 정보 : Korean Circulation Journal / 38권 / 4호 / 212 ~ 219페이지
· 저자명 : 김태균, 김용주, 박찬석, 김동빈, 장성원, 김범준, 정해억, 백상홍, 승기배, 최규보, 박훈준

초록

Background and Objectives: Remodeling of the injured arterial wall is dependent on the action of several extracellular
proteases, including matrix metalloprotease-2 (MMP-2), and this protein is associated with the migration of
vascular smooth muscle cells. The effect of a high dose of external irradiation (20 Gy) on the MMP-2 expression in
neointimal hyperplasia is not known. Materials and Methods: Sprague-Dawley rats were subjected to balloon injury
to the common carotid artery. At 24 hours after injury, 20 Gy external irradiation was done for the irradiated group
(n=25) and this was not done for the control group (n=25). The percent area stenosis, the maximal intimal thickness,
the intima/media area ratio on H-E staining and the MMP-2 positivity on the immunohistochemical staining were
measured. Western blotting and a gelatin zymogram for determining the MMP-2 protein expression were also performed
after the injury. Results: The parameters of neointimal hyperplasia such as the percent area stenosis, the
maximal intimal thickness and the intima/media area ratio were 40.2±12.1%, 0.30±0.12 mm and 1.27±0.32,
respectively, at 14 days after injury, and these parameters were maintained as a hyperplastic state at 28 days after injury
in the control group. There was undetectable neointimal hyperplasia in the irradiated group compared with the control
group (p<0.01). Western blotting demonstrated an increase in the MMP-2 protein level beginning 2 to 4 days after
injury in the control group, but there was only a transient increase in the MMP-2 level at day 2 after injury in the irradiated
group. The gelatin zymogram and immunohistochemical staining also showed the expression of MMP-2 in the
control group, but not in the irradiated group. Conclusion: These findings suggest the suppressed expression of MMP-
2 is associated with reduced neointimal hyperplasia in the balloon injury-rat model.

영어초록

Background and Objectives: Remodeling of the injured arterial wall is dependent on the action of several extracellular
proteases, including matrix metalloprotease-2 (MMP-2), and this protein is associated with the migration of
vascular smooth muscle cells. The effect of a high dose of external irradiation (20 Gy) on the MMP-2 expression in
neointimal hyperplasia is not known. Materials and Methods: Sprague-Dawley rats were subjected to balloon injury
to the common carotid artery. At 24 hours after injury, 20 Gy external irradiation was done for the irradiated group
(n=25) and this was not done for the control group (n=25). The percent area stenosis, the maximal intimal thickness,
the intima/media area ratio on H-E staining and the MMP-2 positivity on the immunohistochemical staining were
measured. Western blotting and a gelatin zymogram for determining the MMP-2 protein expression were also performed
after the injury. Results: The parameters of neointimal hyperplasia such as the percent area stenosis, the
maximal intimal thickness and the intima/media area ratio were 40.2±12.1%, 0.30±0.12 mm and 1.27±0.32,
respectively, at 14 days after injury, and these parameters were maintained as a hyperplastic state at 28 days after injury
in the control group. There was undetectable neointimal hyperplasia in the irradiated group compared with the control
group (p<0.01). Western blotting demonstrated an increase in the MMP-2 protein level beginning 2 to 4 days after
injury in the control group, but there was only a transient increase in the MMP-2 level at day 2 after injury in the irradiated
group. The gelatin zymogram and immunohistochemical staining also showed the expression of MMP-2 in the
control group, but not in the irradiated group. Conclusion: These findings suggest the suppressed expression of MMP-
2 is associated with reduced neointimal hyperplasia in the balloon injury-rat model.

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