실험적 간섬유화 병변의 미세혈관계 변화에 관한 주사 전자현미경적 연구 (Scanning Electron Microscopic Study for Morphological Changes of Microvasculature in Experimental Hepatic Fibrosis)

서지정보

· 발행기관 : 고신대학교(의대) 고신대학교 의과대학 학술지
· 수록지 정보 : 고신대학교 의과대학 학술지 / 19권 / 1호 / 19 ~ 25페이지
· 저자명 : 허방, 이명종, 천봉권, 신동훈, 이충한

초록

Background : The liver parenchymal cells are supported by delicate sinusoidal vessels and portal tract connective tissue. Hepatic fibrosis causes disorganization of these supporting structures, which lead to abnormal microcirculation.
Material & Methods : Morphological changes of hepatic microvasculature were examined by scanning electron microscopy (SEM) in N-diethylnitrosamine (DEN)-induced experimental hepatic fibrosis of rat. Experimental animal livers were perfused with warm saline and fixed with mixture of 0.5% glutaraldehyde and 0.5% paraformaldehyde. And then, methacrylate resin (Mercox CL-2R) was injected through the hepatic artery. Vascular casts were prepared with 20% NaOH maceration.
Results : Hepatic fibrosis with nodular regeneration was seen after 9 week DEN treatment. Microvasculature of DEN-induced liver tissues showed marked distortion and dilatation of major branches of hepatic arteries and portal veins. These changes were initially noticed in 6 week DEN-treated group. Abnormal connection between increased peribiliary plexus (PBP) and branches of hepatic artery and portal veins, and between PBP and sinusoidal vasculature were frequently observed around regenerating nodules.
Conclusions : These findings suggest that development of abnormal microvasculature, especially in PBP and arteriolo-portal anastomosis, in experimental hepatic fibrosis may potentiate the hepatic fibrosis and play a role for micro-collateral circulation in portal hypertension of liver cirrhosis.

영어초록

Background : The liver parenchymal cells are supported by delicate sinusoidal vessels and portal tract connective tissue. Hepatic fibrosis causes disorganization of these supporting structures, which lead to abnormal microcirculation.
Material & Methods : Morphological changes of hepatic microvasculature were examined by scanning electron microscopy (SEM) in N-diethylnitrosamine (DEN)-induced experimental hepatic fibrosis of rat. Experimental animal livers were perfused with warm saline and fixed with mixture of 0.5% glutaraldehyde and 0.5% paraformaldehyde. And then, methacrylate resin (Mercox CL-2R) was injected through the hepatic artery. Vascular casts were prepared with 20% NaOH maceration.
Results : Hepatic fibrosis with nodular regeneration was seen after 9 week DEN treatment. Microvasculature of DEN-induced liver tissues showed marked distortion and dilatation of major branches of hepatic arteries and portal veins. These changes were initially noticed in 6 week DEN-treated group. Abnormal connection between increased peribiliary plexus (PBP) and branches of hepatic artery and portal veins, and between PBP and sinusoidal vasculature were frequently observed around regenerating nodules.
Conclusions : These findings suggest that development of abnormal microvasculature, especially in PBP and arteriolo-portal anastomosis, in experimental hepatic fibrosis may potentiate the hepatic fibrosis and play a role for micro-collateral circulation in portal hypertension of liver cirrhosis.

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