Individual Differences of Ozone Resistance for Seed Germination and Seedling Development of Pinus thunbergii

서지정보

· 발행기관 : 한국농림기상학회
· 수록지 정보 : 한국농림기상학회지 / 12권 / 3호 / 207 ~ 216페이지
· 저자명 : 김두현, 한심희

초록

Individual differences of ozone (O3) resistance for seed production, seed germination and seedling development were examined in this study. Five in each healthy and damaged trees of Pinus thunbergii growing in air polluted area for 12 years were chosen based on visible foliar injury and growth. The cones of P. thunbergii, which were collected from healthy and damaged trees, were analyzed for physical characteristics and seeds from the cones were used to test germination percentage under O3 treatment.
The germinated seeds were continuously exposed to O3 treatment and the lipid peroxidation and activities of antioxidative enzymes were determined for both seeds and seedlings. The O3 treatment for seed germination and seedling development were conducted at three conditions: control, 150 ppb and 300 ppb of O3. The non-treated seeds from the damaged trees showed 21.6% lower germination than those from the healthy ones. On the O3 treatment of 300 ppb, seed germination decreased approximately 10% for the healthy trees and 19% for the damaged trees compared to that on the control. The seeds from the healthy trees showed significantly higher activities of superoxide dismutase (SOD), glutathione reductase (GR),and catalase (CAT) than those from the damaged trees. The activities of GR, ascorbate peroxidase (APX),and CAT decreased along with the increasing O3 concentration in two tree grades. Malondialdehyde (MDA) content of seeds was not influenced by O3 treatment for two tree grades. In seedling development,there were no significant differences for length and biomass of needle and root of two tree grades at both the control and 150 ppb of O3. At 300 ppb of O3 treatment, however, the length and biomass of needle and stem decreased for two tree grades but no significant differences was detected in root. The seedlings from the damaged trees were more sensitive to the O3 treatment, showing higher activities of SOD, APX, and CAT and content of MDA compared to those from the healthy tree seedlings. Our results indicate that seed germination and seedling development are vulnerable to increasing O3 concentrations and that attention must be paid to the individual selection of tree species for reforestation.

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