Cyclic Micropropagation of Phalaenopsis Using Thin Cross Section of Floral Stalk-derived Leaf

서지정보

· 발행기관 : 한국원예학회
· 수록지 정보 : 원예과학기술지 / 27권 / 1호 / 150 ~ 155페이지
· 저자명 : Khin Thida Myint, 김창길, 정미영, 박재석, 임기병, 정재동

초록

An efficient and reproducible procedure has been developed for the rapid in vitro micropropagation of Phalaenopsis by using thin cross sections (TCSs) of leaf explants derived from in vitro flower stalk nodes. Direct induction of multiple shoot-buds was observed from thin sectioned leaf explants cultured on half-strength Murashige and Skoog (MS) medium supplemented with 9.08 µM TDZ under 2 weeks of darkness followed by 16-hr photoperiod under 55 μmol･m-1･s-1 PPFD. Multiple shoot-buds formation ability was totally dependent on the genotype. Leaf explants with multiple shoot-buds were transferred to half-strength MS hormone-free medium for further growth and development. Subsequent multiple shoots formation also occurred at the basal parts of the shoots derived from multiple shoot-buds. The highest number of shoots was observed on half-strength MS medium containing 4.54 µM TDZ in Phalaenopsis cv. Queen Emma and 9.08 µM TDZ in WR. Regenerated shoots rooted on half-strength MS medium supplemented with 5.37 µM NAA and 0.46 µM kinetin were successfully acclimatized and grew into normal plants in a greenhouse.

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