잔디류 돌연변이 유기를 위한 적정 방사선 선량범위의 결정 (Determination of the Optimum Dose Range for Mutation Induction by a Gamma-Ray on Turfgrasses)

서지정보

· 발행기관 : 한국잔디학회
· 수록지 정보 : Weed & Turfgrass Science / 22권 / 1호 / 25 ~ 34페이지
· 저자명 : 이혜정, 이긍주, 김동섭, 김진백, 구자형, 강시용

초록

This study was conducted to determine the optimum dose ranges for a mutation breeding based on the observation of seed germination and early growth in turfgrasses. Three warm season (Zoysiagrass, Bermudagrass, and Seashore paspalum) and four cool season turfgrasses (Kentucky bluegrass, Tall fescue, Perennial ryegrass, and Creeping bentgrass) were used in this study. We investigated the percentage of seed germination and seedling growth after irradiating on turfgrass seeds with various doses of a gamma-ray (100, 200, 300, 400, and 500 Gy). After 24 h with the gamma irradiation, the seedswere sown on the wet filter paper in a petri dish and maintained for 3 weeks at 30?for the warm season turfgrasses and at 25? for cool season turfgrasses. Data for seed germination and seedling growth with three replications were collected. The percentage of seed germination was decreased with increasing of a gamma-ray dose. Shoot and root growth,and fresh weight were significantly decreased as the radiation dose increased. A radiation dose indicating 50% growth inhibition (LD50) with a gamma irradiation was varied among those turfgrass species used, with the highest at about 500 Gy for bermudagrass and the lowest at 100Gy for tall fescue. The optimum dose of a gamma irradiation for selection of turfgrass mutants was considered about 300, 150, 500, 150, 200, 100 and 200 Gy for zoysiagrass, seashore paspalum, bermudagrass, Kentucky bluegrass, perennial ryegrass, tall fescue, and creeping bentgrass, respectively.

영어초록

This study was conducted to determine the optimum dose ranges for a mutation breeding based on the observation of seed germination and early growth in turfgrasses. Three warm season (Zoysiagrass, Bermudagrass, and Seashore paspalum) and four cool season turfgrasses (Kentucky bluegrass, Tall fescue, Perennial ryegrass, and Creeping bentgrass) were used in this study. We investigated the percentage of seed germination and seedling growth after irradiating on turfgrass seeds with various doses of a gamma-ray (100, 200, 300, 400, and 500 Gy). After 24 h with the gamma irradiation, the seedswere sown on the wet filter paper in a petri dish and maintained for 3 weeks at 30?for the warm season turfgrasses and at 25? for cool season turfgrasses. Data for seed germination and seedling growth with three replications were collected. The percentage of seed germination was decreased with increasing of a gamma-ray dose. Shoot and root growth,and fresh weight were significantly decreased as the radiation dose increased. A radiation dose indicating 50% growth inhibition (LD50) with a gamma irradiation was varied among those turfgrass species used, with the highest at about 500 Gy for bermudagrass and the lowest at 100Gy for tall fescue. The optimum dose of a gamma irradiation for selection of turfgrass mutants was considered about 300, 150, 500, 150, 200, 100 and 200 Gy for zoysiagrass, seashore paspalum, bermudagrass, Kentucky bluegrass, perennial ryegrass, tall fescue, and creeping bentgrass, respectively.

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