팽이버섯 재배 현장에서 Listeria monocytogenes의 성장을 억제하기 위한 살균 처리 기술 개발

서지정보

· 발행기관 : 한국식품위생안전성학회
· 수록지 정보 : 한국식품위생안전성학회지 / 38권 / 6호
· 저자명 : 박경민, 이수빈, 정도영, 최송이, 황인준, 김세리

목차

Materials and Methods
실험 균주
팽이버섯추출물 준비
살균소독제 준비
권지 접착포 표면 균주 접종 및 살균소독제 처리 효과
살균소독제 농도별 처리시간 결정
통계처리
Results and Discussion
화학적살균제에 의한 Listeria monocytogenes 저감 효과
유기물 함유에 따른 화학적 살균제의 Listeriamonocytogenes 저감 효과
미산성전해수 처리 농도와 시간에 따른 Listeriamonocytogenes 저감 효과
이산화염소수 처리 농도와 시간에 따른 Listeriamonocytogenes 저감 효과
차아염소산나트륨 처리 농도와 시간에 따른 Listeriamonocytogenes 저감 효과
과산화초산 처리 농도와 시간에 따른 Listeriamonocytogenes 저감효과
Acknowedgement
국문요약
Conflict of interests
ORCID

영어초록

The consumption of enoki mushrooms has been associated with cases of listeriosis produced by Listeria monocytogenes, highlighting the significance of sanitizing food-contact surface, such as the velcro used in welding processing of enoki mushrooms, to ensure microbial safety. We investigated the inhibitory activity of nine chemical disinfectants at regular concentrations against L. monocytogenes isolated from a mushroom farm environment. The bacterial suspension was prepared in phosphate buffered saline and mushroom extract broth and inoculated onto the velcro surface. After inoculation, most disinfectants reduced the initial 8 log CFU/coupon concentration by less than 2 log CFU/coupon during a 5-min treatment. Slightly acidic hypochlorous water showed a reduction of approximately 4 log CFU/coupon when tested for more than 30 min at the maximum allowable concentration of 200 mg/L. Sodium hypochlorite solution showed a reduction of approximately 5 log CFU/coupon when used at 100 mg/L for 60 min. Peracetic acid, at the maximum allowable concentration of 300 mg/L, showed the most effective reduction of 5 log CFU/coupon or more when the surface was treated with 37.5 mg/L for 30 min. These results indicate that peracetic acid can be used as the disinfectant strategy to control cross-contamination of L. monocytogenes on the velcro surface of plastic wrappers used in the welding processing of enoki mushrooms.

참고자료

· 없음