들깨 가식부위별 최적 추출 조건 확립을 위한 항산화 및 항염증 활성 평가

서지정보

· 발행기관 : 한국식품영양학회
· 수록지 정보 : 한국식품영양학회지 / 36권 / 6호
· 저자명 : 김민영, 김정인, 김상우, 김성업, 오은영, 이정은, 이은수, 안연주, 이명희, 김춘송

목차

서 론
재료 및 방법
1. 실험재료 및 추출물 제조
2. 총 폴리페놀 함량 측정
3. 총 플라보노이드 함량 측정
4. ABTS 라디칼 소거능 측정
5. DPPH 라디칼 소거능 측정
6. 세포배양 및 독성 측정
7. 산화질소(nitric oxide) 및 염증성 사이토카인(cytokine)분석
8. 통계분석
결과 및 고찰
1. 총 폴리페놀 및 플라보노이드 함량
2. 항산화 활성
3. 항염증 활성
4. 상관관계분석
요약 및 결론
감사의 글
References

영어초록

This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25°C), shaking extraction (SHE, 80°C), and sonication assisted extraction(SAE, , 25°C) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.

참고자료

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