Establishment of withdrawal time and analysis of amprolium residue in edible tissues of broiler chickens administered in drinking water

서지정보

· 발행기관 : 한국예방수의학회(구 한국수의공중보건학회)
· 수록지 정보 : 예방수의학회지 / 47권 / 2호
· 저자명 : Jun Young Kim, Woo H. Kim, Yeung Bae Jin, Kwang Il Park, Sung Joong Lee, Suk Kim, Hu-Jang Lee

목차

서 론
재료 및 방법
시약 및 재료
실험동물
시험약제 투여 및 시료채취
표준원액 및 표준용액의 조제
시료 전처리
기기분석조건
분석법 검증
육계 조직에 대한 APL의 잔류휴약기간 설정
결 과
APL의 크로마토그램
검량곡선, 검출한계 및 정량한계
회수율과 정밀성
육계 조직 중 APL 잔류 분석
육계 조직에 대한 APL의 잔류휴약기간 설정
고 찰
감사의 글

영어초록

From 2020, Korean Animal and Plant Quarantine Agency has reset the withdrawal time (WT) for veterinary drugs typically used in livestock in preparation for the introduction of positive list system (PLS) program in 2024. This study was conducted to reset the MRL for amprolium (APL) in broiler chickens as a part of PLS program introduction. Forty-eight healthy Ross broiler chickens were orally administered with APL at the concentration of 60 mg/L (APL-1, n=24) for 14 days and 240 mg/L (APL-2, n=24) for 7 days through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3 and 5 days, respectively. Residual APL concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 0.3~5.0 μg/kg), recoveries (81.5~112.4%), and coefficient of variations (<15.5%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In APL-1, APL in all tissues except for kidney was detected less than LOQ at 3 days after drug treatment. In APL-2, APL in liver and kidney was detected more than LOQ at 5 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal periods of APL-1 and APL-2 in poultry tissues were established to 3 and 2 days, respectively. In conclusion, the developed analytical method is sensitive and reliable for detecting APL in poultry tissues. The estimated WT of APL in poultry tissues is longer than the current WT recommendation of 2 days for APL in broiler chickens.

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