Establishment of withdrawal time and analysis of ethopabate residue in edible tissues of broiler chickens administered in drinking water

서지정보

· 발행기관 : 한국예방수의학회(구 한국수의공중보건학회)
· 수록지 정보 : 예방수의학회지 / 47권 / 3호
· 저자명 : Jun Young Kim, Yeung Bae Jin, Woo H. Kim, Kwang Il Park, Sung Joong Lee, Suk Kim, Hu-Jang Lee

목차

서 론
재료 및 방법
시약 및 재료
실험동물
시험약제 투여 및 시료채취
표준원액 및 표준용액의 조제
시료 전처리
기기분석조건
분석법 검증
육계 조직에 대한 EPB의 잔류휴약기간 설정
결 과
EPB의 크로마토그램
검량곡선, 검출한계 및 정량한계
회수율과 정밀성
육계 조직 중 EPB 잔류 분석
육계 조직에 대한 EPB의 잔류휴약기간 설정
고 찰
감사의 글

영어초록

This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.

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